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Structural characterization of immune signaling protein TIGIT using x-ray crystallography and cryo-electron microscopy

Objective

The goal of this proposal is to obtain atomic resolution structures of TIGIT using X-ray crystallography and cryo-electron microscopy. TIGIT inhibits autoimmune responses, and anti-TIGIT antibodies can induce immune response in anti-viral and anti-tumor immunotherapies. No structures exist for the transmembrane (TM) domain of TIGIT, which is the portion of the signaling protein that transmits signals accross the membrane bilayer, and there are no structures of TIGIT bound to anti-TIGIT antibodies. High-resolution structures of full-length TIGIT and TM domain constructs will further our understanding of how TIGIT transmits signals across the cell membrane and modulates T cell activation. Structures of TIGIT TM constructs bound to PVR and anti-TIGIT antibodies will guide the design of anti-TIGIT drugs and antibodies for use in immunotherapy treatments. The candidate will carry out this structural work in the lab of Prof. Martin Caffrey, a world- renowned leader in the field of membrane protein X-ray crystallography.

Fields of science (EuroSciVoc)

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Programme(s)

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Topic(s)

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Funding Scheme

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MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF)

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Call for proposal

Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.

(opens in new window) H2020-MSCA-IF-2017

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Coordinator

THE PROVOST, FELLOWS, FOUNDATION SCHOLARS & THE OTHER MEMBERS OF BOARD, OF THE COLLEGE OF THE HOLY & UNDIVIDED TRINITY OF QUEEN ELIZABETH NEAR DUBLIN
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 175 866,00
Address
COLLEGE GREEN TRINITY COLLEGE
D02 CX56 Dublin
Ireland

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Region
Ireland Eastern and Midland Dublin
Activity type
Higher or Secondary Education Establishments
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

€ 175 866,00
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