Objective
Pre-mRNA splicing is the major step of RNA edition in eukaryotes. It consists on a catalytic process that removes non-coding sequences (introns) and ligates the coding ones (exons), creating the RNA sequence that codifies the final protein. This process is highly regulated and gives rise to a great variability of proteins, expanding the versatility encoded in the human genome. In the early stage of splicing, RNA 5’ and 3’ splice sites must be brought within proximity to correctly assemble the active spliceosome (cross-intron arrangement) and perform the excision and ligation reactions. Many elements participate in the assembly of the early spliceosomal complex (E complex) through different protein-protein, protein-RNA and RNA-RNA interactions, leading to the formation of a very dynamic association. Several factors regulate the correct assembly, defining the RNA sequences that should be excised and controlling the production of alternative spliced transcripts. The full comprehension of the E complex formation will allow a deeper understanding of the splicing mechanism at molecular level which is essential in human health as aberrant processes are the basis of many diseases.
Currently, the structures of cross-intron arrangement factors in the early spliceosome are not known and there is a lack of structural data of the complete E complex. In this project, I propose a novel, integrative and multidisciplinary study of the structure and regulation of the early spliceosomal assembly. I will analyse the different cross-intron interactions, elucidate the high-resolution structure of the whole association and study the effects of protein post-translational modifications in the structure and complex assembly. This will lead to the discovery of new structural insights for the first step of the spliceosome formation having an impact on our understanding of the fundamental mechanism of splicing regulation and forming the basis for future innovative therapeutic approaches.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences biological sciences genetics RNA
- natural sciences biological sciences genetics genomes
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions
MAIN PROGRAMME
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H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility
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Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
MSCA-IF-EF-ST - Standard EF
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Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) H2020-MSCA-IF-2017
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Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
85764 Neuherberg
Germany
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.