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Epithelial/Mesenchymal Cross-talk in response to injury and early tumorigenesis; a spatiotemporal perspective

Objective

Epithelial tissues have evolutionarily adapted to rapidly react to tissue disruption in order to restore the epithelial barrier and ensure survival. The dynamic behaviour of epithelial cells in response to injury represents a decisive mechanism to recover tissue integrity. Following injury, not only cycling but also differentiating epithelial cells are able to revert back, reacquiring stem cell-like behaviour and contributing to tissue regeneration. Although this cellular plasticity is normally a strictly controlled and limited process, if inadequately activated in cells with pre-existing tumour-initiating mutations it will have implications for cancer development.
In this proposal, I will investigate the little-known mechanisms governing the dynamic changes in epithelial cell behaviour in the events after tissue injury using the uncomplicated architecture of the oesophagus as a model. The aim is to identify molecular regulators governing cell fate switching following the early wound response, and ultimately, reveal their potential relevance for early epithelial tumour formation. Given the increasing relevance of the mesenchymal compartment both in tissue maintenance and disease, I will particularly focus on the contribution of epithelial-mesenchymal cross-talk in the regulation of epithelial fate changes.
To this end, I present an innovative approach that combines precise lineage tracing cell fate data with spatiotemporal single-cell whole transcriptomics. This approach relies on cutting-edge techniques and transgenic animal models widely established in the host laboratory that, when combined, will enable the identification of rules regulating behaviour of epithelial cells with temporal and spatial resolution.

Fields of science (EuroSciVoc)

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Programme(s)

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Topic(s)

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Funding Scheme

Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.

MSCA-IF-EF-ST - Standard EF

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Call for proposal

Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.

(opens in new window) H2020-MSCA-IF-2017

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Coordinator

THE CHANCELLOR MASTERS AND SCHOLARS OF THE UNIVERSITY OF CAMBRIDGE
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 195 454,80
Address
TRINITY LANE THE OLD SCHOOLS
CB2 1TN CAMBRIDGE
United Kingdom

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Region
East of England East Anglia Cambridgeshire CC
Activity type
Higher or Secondary Education Establishments
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

€ 195 454,80
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