Periodic Reporting for period 1 - SOLO (Single cell omics of the Oligodendrocyte Lineage in develOpment and disease)
Reporting period: 2018-06-01 to 2020-05-31
There is a need for a detailed characterization of these oligodendroglia populations and the standard use of computational methods to study the highly complex single cell data in a reproducible manner. Mouse based studies showed a heterogeneity in oligodendroglia populations in both development and mouse MS models like Experimental autoimmune encephalomyelitis (EAE). The completion of the SOLO MSCA led to the implementation and to share methodology for the study of oligodendroglia in development and MS. The results also include web base open access to data. Moreover, the results using brains from MS patients for the first time using single cell technologies open new perspectives in the knowledge of the disease in patients and the relevance of oligodendroglia in MS (Jäckel and Agirre et al. Nature 2019). The results also showed a preliminary new classification of the MS lesion types based on transcriptional profiles complementary to the histopathological classification.
The objectives of this project include;
1) Correlate single cell transcriptomics and epigenomics data in healthy states and disease; 2) Establish specific OL lineage classes and its links to MS; 3) Identify specific regulatory pathways determining the healthy and disease populations.
The results from this action have been communicated in international conferences and are present as several high open green access publications. Moreover, results from the snRNA-seq study of skewed oligodendrocyte heterogeneity in MS was selected as Scientific highlights at the European Committee for Treatment and Research in Multiple Sclerosis (ECTRIMS) Congress 2019.
The computational and methodological aspect of the action has resulted in openly shared code and data that it has been accessed through repositories like Github by the scientific and computational community. Advances in computational biology and more specifically single cell methodologies require a fast development of methodology to address the challenges on biomedicine. The objectives completed with this action are and will be clue for the advances in the study of demyelinating and neurodegenerative diseases that have high impact on the society. Follow up research projects are expected from the results and resources generated during the project.