AstroMiRimage is split into six specific Work Packages (WPs), three for research, one for communication, dissemination and exploitation, one for training/transfer of knowledge, and one for management of the project.
Through research in WPs 1, 2 and 3, the Fellow has isolated microRNAs (short non-coding RNAs that regulate gene expression post-transcriptionally by targeting mRNAs and preventing their translation) from synaptosomes. Synapses are the contact points between two neurons, and the majority of synapses across the brain are ensheathed by astrocytic processes. Targeting these microRNAs will influence astrocyte morphology and local translation which might allow the clearing of excess glutamate and potassium from the synaptic cleft and hence prevent seizure generation.
Throughout the project, the Fellow acquired training in several state-of-the-art techniques, including modelling epilepsy in mice, RNA isolation and sequencing, and super-resolution microscopy imaging.
In WP4, the Fellow communicated project findings via oral and poster presentations at nine national and international conferences and meetings. Furthermore, the Fellow participated in four public outreach activities to promote AstroMiRimage and to communicate findings to the general public.
In WP5, the Fellow gained transferable skills attending ten training workshops and multi-day conferences. The Fellow conducted research workshops with hands-on experience for researchers and provided supervision and mentoring for early career researchers and undergraduate students.
The impact of AstroMiRimage is further highlighted through the publication of three Open Access peer-reviewed articles in high impact journals, one article under review, and one article under preparation. The findings obtained over the course of this Fellowship will contribute to disease modifying therapies and improve the lives of people with epilepsy.
