Objective
The successful implementation of the common plant health policy in the European Community is largely dependent upon the availability of reliable test procedures for high risk statutory organisms. Essentially standardized methods for laboratory tests which are harmonized in all Member States are a prerequisite.
Recent advances in molecular biology have generated powerful fingerprint methods for rapid, specific and sensitive detection and identification of plant pathogens with obvious advantages over the common conventional methods, in particular serological tests.
The project aims to introduce these technologies in methods for reliable test-monitoring of the bacteria under the EC Plant Health Directives.
The successful implementation of the common plant health policy is the European Community is largely dependent on the availability of reliable test procedures for high risk statutory organisms. It is necessary to have standardized methods which are harmonized in all Member States. Recent advances in molecular biology have generated powerful fingerprint methods for rapid, specific and sensitive detection and identification of plant pathogens with obvious advantages over the common conventional methods, in particular serological tests. Work is being done to improve the detection, diagnosis and identification of bacteria which are quarantine or high risk statutory organisms by the development and exploitation of nucleic acid probe techniques.
Probes are now available for 5 quarantine bacteria. Protocols were prepared for deoxyribonucleic acid (DNA) hybridization tests for 2 organisms, ring tests made with bacterial cell suspensions and hybridization conditions, labelling and detection systems evaluated. Protocols have been evaluated for colony hybridization tests and direct blotting and ring tests made with bacterial cell suspensions and contaminated plant material. Primers were prepared for polymerase chain reaction (PCR) amplification tests, reagents distributed and the first round of tests made for sensitivity.
The programme consists of (1) testing of probes in dot blot hybridization, (2) development and quantification of target DNA amplification by the Polymerase Chain Reaction (PCR), (3) identification and genetic characterization in Restriciton Fragment Length Polymorphism (RFLP) analysis and (4) rapid detection of single cells with fluorescent RNA oligonucleotide probes.
The work consists of the preparation of the test protocols and experiments to evaluate the methods, which are performed in three consecutive stages :
1) testing of cell suspensions of the target bacteria, related and non-related pathogenic and saprophytic bacteria, in particular those naturally present in association with the plant material,
2) reconstruction tests with artificially contaminated plant extracts,
3) ringtests using samples plant material with different levels of natural contamination.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- agricultural sciences agriculture, forestry, and fisheries agriculture agronomy plant protection
- natural sciences biological sciences biochemistry biomolecules nucleic acids
- natural sciences biological sciences microbiology bacteriology
- natural sciences biological sciences genetics DNA
- natural sciences biological sciences molecular biology
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Programme(s)
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Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
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Coordinator
9820 MERELBEKE
Belgium
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.