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The regulatory GENomE of SWine and CHicken: functional annotation during development

CORDIS provides links to public deliverables and publications of HORIZON projects.

Links to deliverables and publications from FP7 projects, as well as links to some specific result types such as dataset and software, are dynamically retrieved from OpenAIRE .

Deliverables

Availability of software implementing the proposed Bayesian genomic prediction model incorporating functional annotations

Proposed method documented and source-code available on GitHub (Software).

Raw sequences of ChiP-seq and ATAC-seq delivered to ENA and FAANG

fastq files and metadata available at FAANG and ENA.

Raw sequences of Hi-C Capture delivered to ENA and FAANG

fastq files and metadata available at FAANG and ENA.

Reproducible, scale-able workflows for FAANG data analysis

We will release on GitHub workflows that are 100% reproducible and scale-able from laptop to HPC to cloud, using a workflow language such as Snakemake or NextFlow, integrating best-in-class bioinformatics open-source software

Raw sequences of WGBS and RRBS delivered to ENA and FAANG

fastq files and metadata available at FAANG and ENA.

Raw sequences of RNA-seq delivered to ENA and FAANG

fastq files and metadata available at FAANG and ENA.

Bio-repository of 3120 samples

A list of 3120 samples corresponding to 10 aliquots * 13 tissues * 4 replicates * 3 developmental stages * 2 species

Fully functional data coordination platform for production use

Production release of the data coordination platform for the validation enrichment and submission of GENESWitCH sample experiment and analysis data to FAANG through the European public archives The preliminary system will already have been heavily tested optimised and utilised from sample and raw data will be submitted steadily from the start of the project This final version will contain the full configuration of the submission process for the requirements of work packages 1 2 4 and 5

Visualisation and API access for improved annotation in chicken and pig

Programmatic access to the underlying data in Ensembl genomes is made available via the Ensembl API, which enables others to build software and services based on public Ensembl data.

Advanced Bioinformatics Trainings

Organize two trainings of two days each focussing on the genomic prediction models that will be developed within the project.

GENE-SWitCH project data portal presentation platform

A customised GENE-SWitCH section of the FAANG data portal (http://data.faang.org/) with pre-filtered tabulated view of GENE-SWitCH generated samples, datasets and analyses. The platform will include innovative presentation displays of GENE-SWitCH analysis tracks, workflows stored in GitHub and the sampling and experimental protocols developed within the project.

Improved chicken annotation, including variant and transcript annotations improvements

A new chicken genome annotation will be released that includes transcript (coding and non-coding) and gene annotation, regulatory regions, location and likely effect of variants, comparative genomics across hundreds of vertebrate species, and tissue- and time-point specific annotations.

Webinar

An interactive webinar will be organized, focussing on the value of genetic information – including richly annotated high-quality genome sequences.

Improved pig annotation, including variant and transcript annotations improvements

A new pig genome annotation will be released that includes transcript (coding and non-coding) and gene annotation, regulatory regions, location and likely effect of variants, comparative genomics across hundreds of vertebrate species, and tissue- and time-point specific annotations.

Report of communication materials

Project website, social media, newsletters (8 issues) and two brochures will be developed.

Publication on state of the art of SFS-30 projects, the European node of FAANG DCC and FAANG platform developments

Submission of a publication that covers the state of the art of the SFS-30 projects and their interaction through the European node of the FAANG Data Coordination Centre. It will cover the developments to the FAANG submission platform and the advances in data presentation methods of the FAANG data portal. It will also report on the breadth, diversity and standardisation of the FAANG SFS-30 datasets and the contribution that these make to the FAANG community.

Project Management Guidelines

Report compiling the guidelines and rules in place for the coordination, reporting and quality control of the project.

Report on coordinated data production, recording and archiving within the European node of FAANG DCC

Report on the establishment of the European node of the FAANG Data Coordination Centre through agreement of scope, remit, objectives and priorities between the SFS-30 funded projects. The report will outline the interoperability development work, a basis for sharing of data and analysis methodologies and framework that will lead to cross project comparative analyses.

Outreach, dissemination, and training plan

D6.1 will be used to implement and evaluate the progress of the activities targeted to relevant stakeholders.

Functional annotations across fine-mapped, selected regions contributing to body-weight in the experimental Virginia chicken lines

Twenty loci with known large effect on body weight in the experimental Virginia chicken lines will be fine-mapped, using a large deep intercross mapping population of ~4000 birds, to haplotypes originating from the founder White Plymouth Rock breed.

Empirical validation of improved genomic prediction models in commercial pig populations

Results of the validation of the developed models, and benchmarking against other models, will be described in a scientific publication.

Validation of developed models with simulated or publicly available data

Results of the validation of the developed models and benchmarking against other models will be described in a scientific publication

Integrated analysis of RNA-seq and ATAC-seq data

Results of integrated analyses of two tissues in light of the reference annotation maps (WP3) at these tissues.

Submission of publication on state of the art and usage of ontologies

Publication on state of the art and usage of ontologies within monogastric and wider farm and companion animal communities. WP3 will incorporate findings from the survey of ontology usage and requirements from the monogastric community and reports on the ontological developments and improvements within the project.

Knowledge Exchange Platform recommendations report

Meeting minutes from 4 physical meetings of the Advisory Board will be prepared as a Recommendation Report.

Results of the comparative analyses

Comparisons within and between species released as data tables and tracks on the FAANG track hub

Final Conference proceedings

Project results will be shared through a final conference.

GENE-SWitCH e-book of abstracts

All project results will be shared through an e-book of abstracts.

Empirical validation of improved genomic prediction models in commercial chicken populations

Demonstrate the potential of using annotation information (ie eQTLs and functional information) to improve accuracy of genomic selection through validation in a commercially relevant population.

Significant associations detected by eQTL analysis in pigs

Comprehensive map of eQTL in porcine tissues that are central to whole-body energetic homeostasis: small intestine, liver and muscle.

Operational analysis workflows in Embassy Cloud

Full analysis containerised workflows archived in GitHub and actively processing incoming project data Evidenced by analysis data flowing into FAANG through the European public archives managed by the data coordination platform D32 WP2 will be implementing initial workflows as soon as raw data becomes available By month 24 the operational infrastructure will be in place for analysis results from WP2 WP4 and WP5 to be delivered in the later half of the project

GENE-SWitCH Data Management Plan

Report on the data management plan for GENE-SWitCH in accordance with the latest FAANG data standards, SOPs and FAANG Data Sharing Principles.

Publications

Detailed molecular and epigenetic characterization of the Pig IPECJ2 and Chicken SL-29 cell lines

Author(s): Jani de Vos, Richard P.M.A. Crooijmans, Martijn F.L. Derks, Susan L. Kloet, Bert Dibbits , Martien A.M. Groenen, Ole Madsen
Published in: iScience, 2023, ISSN 2589-0042
Publisher: Cell Press

From FAANG to fork: application of highly annotated genomes to improve farmed animal production

Author(s): Emily L. Clark, Alan L. Archibald, Hans D. Daetwyler, Martien A. M. Groenen, Peter W. Harrison, Ross D. Houston, Christa Kühn, Sigbjørn Lien, Daniel J. Macqueen, James M. Reecy, Diego Robledo, Mick Watson, Christopher K. Tuggle, Elisabetta Giuffra
Published in: Genome Biology, Issue 21/1, 2020, ISSN 1474-760X
Publisher: Springer
DOI: 10.1186/s13059-020-02197-8

The European Nucleotide Archive in 2019

Author(s): Clara Amid, Blaise T F Alako, Vishnukumar Balavenkataraman Kadhirvelu, Tony Burdett, Josephine Burgin, Jun Fan, Peter W Harrison, Sam Holt, Abdulrahman Hussein, Eugene Ivanov, Suran Jayathilaka, Simon Kay, Thomas Keane, Rasko Leinonen, Xin Liu, Josue Martinez-Villacorta, Annalisa Milano, Amir Pakseresht, Nadim Rahman, Jeena Rajan, Kethi Reddy, Edward Richards, Dmitriy Smirnov, Alexey Sokolov, Sen
Published in: Nucleic Acids Research, 2019, ISSN 0305-1048
Publisher: Oxford University Press
DOI: 10.1093/nar/gkz1063

Accounting for overlapping annotations in genomic prediction models of complex traits

Author(s): Mollandin, F., Gilbert, H., Croiseau, P., and Rau, A.
Published in: BMC Bioinformatics, 2022, ISSN 1471-2105
Publisher: BioMed Central
DOI: 10.1186/s12859-022-04914-5

Mutations on a conserved distal enhancer in the porcine C-reactive protein gene impair its expression in liver

Author(s): C. Hernández-Banqué, T. Jové-Juncà, D. Crespo-Piazuelo, O. González-Rodríguez, Y. Ramayo-Caldas, A. Esteve-Codina, M.J. Mercat, M. Bink, R. Quintanilla1, M. Ballester
Published in: Frontiers in Immunology, 2023, ISSN 1664-3224
Publisher: -
DOI: 10.3389/fimmu.2023.1250942

Identification of transcriptional regulatory variants in pig duodenum, liver, and muscle tissues

Author(s): Daniel Crespo-Piazuelo; Hervé Acloque; Olga González-Rodríguez; Mayrone Mongellaz; Marie-José Mercat; Marco C A M Bink; Abe E Huisman; Yuliaxis Ramayo-Caldas; Juan Pablo Sánchez; Maria Ballester
Published in: GigaScience, Issue 18, 2023, Page(s) 1-14, ISSN 2047-217X
Publisher: Oxford University Press
DOI: 10.1093/gigascience/giad042

TAGADA: a scalable pipeline to improve genome annotations with RNA-seq data

Author(s): Cyril Kurylo, Cervin Guyomar, Sylvain Foissac, Sarah Djebali
Published in: NAR Genomics and Bioinformatics, Issue 5, 2024, ISSN 2631-9268
Publisher: Oxford
DOI: 10.1093/nargab/lqad089

Ensembl 2024

Author(s): Peter W Harrison, M Ridwan Amode, Olanrewaju Austine-Orimoloye, Andrey G Azov, Matthieu Barba, If Barnes, Arne Becker, Ruth Bennett, Andrew Berry, Jyothish Bhai, Simarpreet Kaur Bhurji, Sanjay Boddu, Paulo R Branco Lins, Lucy Brooks, Shashank Budhanuru Ramaraju, Lahcen I Campbell, Manuel Carbajo Martinez, Mehrnaz Charkhchi, Kapeel Chougule, Alexander Cockburn, Claire Davidson, Nishadi H De Silva,
Published in: Nucleic Acids Research, 2023, ISSN 1362-4962
Publisher: Oxford University Press
DOI: 10.1093/nar/gkad1049

Ensembl 2022

Author(s): Fiona Cunningham, James E Allen, Jamie Allen, Jorge Alvarez-Jarreta, M Ridwan Amode, Irina M Armean, Olanrewaju Austine-Orimoloye, Andrey G Azov, If Barnes, Ruth Bennett, Andrew Berry, Jyothish Bhai, Alexandra Bignell, Konstantinos Billis, Sanjay Boddu, Lucy Brooks, Mehrnaz Charkhchi, Carla Cummins, Luca Da Rin Fioretto, Claire Davidson, Kamalkumar Dodiya, Sarah Donaldson, Bilal El Houdaigui, Tama
Published in: Nucleic Acids Research, 2022, ISSN 0305-1048
Publisher: Oxford University Press
DOI: 10.1093/nar/gkab1049

The FAANG Data Portal: Global, Open-Access,“FAIR”, and Richly Validated Genotype to Phenotype Data for High-Quality Functional Annotation of Animal Genomes

Author(s): Peter W Harrison, Alexey Sokolov, Akshatha Nayak, Jun Fan, Daniel Zerbino, Guy Cochrane, Paul Flicek
Published in: Frontiers in Genetics, Issue 12 2021/6/17, 2021, Page(s) 873, ISSN 1664-8021
Publisher: Frontiers Media
DOI: 10.3389/fgene.2021.639238

Ensembl 2021

Author(s): Kevin L Howe, Premanand Achuthan, James Allen, Jamie Allen, Jorge Alvarez-Jarreta, M Ridwan Amode, Irina M Armean, Andrey G Azov, Ruth Bennett, Jyothish Bhai, Konstantinos Billis, Sanjay Boddu, Mehrnaz Charkhchi, Carla Cummins, Luca Da Rin Fioretto, Claire Davidson, Kamalkumar Dodiya, Bilal El Houdaigui, Reham Fatima, Astrid Gall, Carlos Garcia Giron, Tiago Grego, Cristina Guijarro-Clarke, Lea
Published in: Nucleic Acids Research, Issue 49/D1, 2020, Page(s) D884-D891, ISSN 0305-1048
Publisher: Oxford University Press
DOI: 10.1093/nar/gkaa942

Copy number variation on ABCC2-DNMBP loci impacts the diversity and composition of the gut microbiota in pigs

Author(s): Y. Ramayo-Caldas, D. Crespo-Piazuelo, J. Morata, O. González-Rodríguez, C. Sebastià, A. Castello, A. Dalmau, S. Ramos-Onsins, K.G. Alexiou, J.M. Folch, R. Quintanilla, M. Ballester
Published in: Microbiology Spectrum, 2023, ISSN 2165-0497
Publisher: -
DOI: 10.1128/spectrum.05271-22

Adding gene transcripts into genomic prediction improves accuracy and reveals sampling time dependence

Author(s): Bruno C Perez; Marco C A M Bink; Karen L Svenson; Gary A Churchill; Mario P L Calus
Published in: G3 Genes|Genomes|Genetics, Issue 17, 2022, Page(s) 12; 11; 2022, ISSN 2160-1836
Publisher: Genetics Society of America
DOI: 10.1093/g3journal/jkac258

Ensembl 2023

Author(s): Fergal J Martin, M Ridwan Amode, Alisha Aneja, Olanrewaju Austine-Orimoloye, Andrey G Azov, If Barnes, Arne Becker, Ruth Bennett, Andrew Berry, Jyothish Bhai, Simarpreet Kaur Bhurji, Alexandra Bignell, Sanjay Boddu, Paulo R Branco Lins, Lucy Brooks, Shashank Budhanuru Ramaraju, Mehrnaz Charkhchi, Alexander Cockburn, Luca Da Rin Fiorretto, Claire Davidson, Kamalkumar Dodiya, Sarah Donaldson, Bilal
Published in: Nucleic Acids Research, Issue 18, 2022, ISSN 1362-4962
Publisher: Oxford University Press
DOI: 10.1093/nar/gkac958

Genomic architecture of carcass and pork traits and their association with immune capacity

Author(s): T. Jové-Juncà, D. Crespo-Piazuelo, O. González-Rodríguez, M. Pascual, C. Hernández-Banqué, J. Reixach, R. Quintanilla, M. Ballester
Published in: Animal, 2023, ISSN 1751-7311
Publisher: Cambridge University Press
DOI: 10.1016/j.animal.2023.101043

The European Nucleotide Archive in 2020

Author(s): Peter W Harrison, Alisha Ahamed, Raheela Aslam, Blaise T F Alako, Josephine Burgin, Nicola Buso, Mélanie Courtot, Jun Fan, Dipayan Gupta, Muhammad Haseeb, Sam Holt, Talal Ibrahim, Eugene Ivanov, Suran Jayathilaka, Vishnukumar Balavenkataraman Kadhirvelu, Manish Kumar, Rodrigo Lopez, Simon Kay, Rasko Leinonen, Xin Liu, Colman O’Cathail, Amir Pakseresht, Youngmi Park, Stephane Pesant, Nadim Rahm
Published in: Nucleic Acids Research, Issue 49/D1, 2020, Page(s) D82-D85, ISSN 0305-1048
Publisher: Oxford University Press
DOI: 10.1093/nar/gkaa1028

An evaluation of the predictive performance and mapping power of the BayesR model for genomic prediction

Author(s): Mollandin, F., Rau, A., and Croiseau, P.
Published in: G3 Genes|Genomes|Genetics, 2022, ISSN 2160-1836
Publisher: Genetics Society of America
DOI: 10.1093/g3journal/jkab225

Microbial Regulation of Host Physiology by Short-chain Fatty Acids

Author(s): Bart van der Hee, Jerry M. Wells
Published in: Trends in Microbiology, Issue 29, 2021, Page(s) 700-712, ISSN 0966-842X
Publisher: Elsevier BV
DOI: 10.1016/j.tim.2021.02.001

Prediction performance of linear models and gradient boosting machine on complex phenotypes in outbred mice

Author(s): B. C. Perez, M. C. A. M. Bink, K. L. Svenson, G. A. Churchill and M. P. L. Calus
Published in: G3 Genes|Genomes|Genetics, Issue 12; 4; 2022, 2022, Page(s) jkac039, ISSN 2160-1836
Publisher: Genetics Society of America
DOI: 10.1093/g3journal/jkac039

nf-core/isoseq: simple gene and isoform annotation with PacBio Iso-Seq long-read sequencing

Author(s): Sébastien Guizard; Katarzyna Miedzinska; Jacqueline Smith; Jonathan Smith; Richard I Kuo; Megan Davey; Alan Archibald; Mick Watson
Published in: Bioinformatics, Issue 4, 2023, Page(s) , vol. 39 , no. 5 , 24323 pp. 1-2, ISSN 1367-4811
Publisher: Oxford University Press
DOI: 10.1093/bioinformatics/btad150

524. Deciphering genetic variants from whole genome affecting duodenum, liver and muscle transcriptomes in pigs

Author(s): Crespo-Piazuelo, D; González-Rodríguez, O; Mongellaz, M; Acloque, H; Mercat, M-J; Bink, M, C a M; Huisman, A, E; Ramayo-Caldas, Y; Sánchez, J, P; Ballester, M
Published in: World Congress on Genetics Applied to Livestock Production WCGALP 2022, Jul 2022, Rotterdam, Netherlands, Issue 10, 2023
Publisher: Wageningen Academic Publishers
DOI: 10.3920/978-90-8686-940-4

303. Capitalizing on complex annotations in Bayesian genomic prediction for a backcross population of growing pigs

Author(s): F. Mollandin, H. Gilbert, P. Croiseau, A. Rau
Published in: Proceedings of 12th World Congress on Genetics Applied to Livestock Production (WCGALP), Issue 3, 2023, Page(s) 1275-1277
Publisher: Wageningen Academic Publishers
DOI: 10.3920/978-90-8686-940-4_303

550. Extensive functional genomics information from early developmental time points for pig and chicken

Author(s): H. Acloque, P.W. Harrison, W. Lakhal, F. Martin, A.L. Archibald, M. Beinat, M. Davey, S. Djebali, S. Foissac, S. Guizard, C. Guyomar, R. Kuo, C. Kurylo, O. Madsen, K. Miedzinska, M. Mongellaz, J. Smith, J. Smith, A. Sokolov, J. de Vos, E. Giuffra, M. Watson
Published in: Proceedings of 12th World Congress on Genetics Applied to Livestock Production (WCGALP), 2023
Publisher: Wageningen Academic Publishers
DOI: 10.3920/978-90-8686-940-4_550

Editorial: Functional Annotation of Animal Genomes.

Author(s): Amanda Jane Chamberlain, Hans H Cheng, Elisabetta Giuffra, Christa Kuehn, Christopher K Tuggle, Huaijun Zhou
Published in: Frontiers in Genetics, 2021, ISSN 1664-8021
Publisher: Frontiers Media
DOI: 10.3389/fgene.2021.768626

Intégration d'annotations fonctionnelles dans des modèles de prédiction génomique bayésiens

Author(s): Mollandin, F.
Published in: 2022
Publisher: -

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