Project description DEENESFRITPL Innovative carriers for more efficient photodynamic therapy Photodynamic therapy (PDT) involves the use of a drug that is activated by light to kill cancer cells. The presence of oxygen is crucial for radical oxygen species generation, which causes the death of tumour cells. A major obstacle in PDT in cancer is the hypoxia in tumour tissue. Funded by the Marie Skłodowska-Curie Actions programme, the OXIGENATED project aims to develop haemoglobin drug delivery nanocarriers (HDDNs) for the simultaneous oxygen and photosensitiser delivery to tumour tissue for efficient PDT. The HDDNs will transport oxygen complexed to haemoglobin with the photosensitiser molecules entrapped in the core. The uptake of HDDNs by cells as well as their intracellular fate and toxicity will be tested in vitro. In vivo studies on mice will trace radiolabelled carriers using tomography. Show the project objective Hide the project objective Objective A major drawback of Photodynamic Therapy (PDT) and other therapies for cancer treatment is the limited oxygen content, hypoxia, in tumour tissue. In PDT a photosensitizing molecule is delivered to malignant tissue to generate radical oxygen species (ROS). The presence of oxygen is fundamental for ROS generation, ultimately causing the death of tumour cells.This project aims to develop hemoglobin drug delivery nanocarriers in the nano and submicron range for simultaneous oxygen and photosensitizer delivery to tumour tissue for a more efficient Photodynamic Therapy.Hemoglobin-based nanocarriers (HOBCs) will be prepared by co-precipitation of hemoglobin with carbonates and surface coating with bovine serum albumin. The carriers will transport oxygen complexed to hemoglobin while photosensitizer molecules will be entrapped in the core. Carriers will be modified with homing peptides to target them to cancer cells. In vitro studies will be conducted to study the uptake of HOBCs by cells, their intracellular fate, toxicity, and oxygen and photosensitizer delivery. In vivo fate of carriers will be studied in mice with radiolabeled carriers by Positron Emission Tomography and Single Emission Computer Tomography. The efficiency of the HOBCs for oxygen delivery and for PDT will be tested in vitro and in vivo in breast and skin cancer models.A multidisciplinary team has been gathered with scientists at the forefront of Material Science, Self assembly, Physics, Chemistry, Imaging, Molecular Biology and Cancer Therapy from Germany, Estonia, Spain, Brazil, Argentina and Thailand. The participation of a SME will be fundamental for the future commercialization of project developments. OXIGENATED will actively work towards exchanging skills and knowledge through secondments of Early Stage and Experienced Researchers, and through networking and training activities. Seconded researchers will develop new scientific and complementary skills while exposed to new research environments. Fields of science natural scienceschemical sciencesinorganic chemistryinorganic compoundsnatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsmedical and health sciencesclinical medicineoncologyengineering and technologyother engineering and technologiesmicrotechnologymolecular engineeringnatural sciencesbiological sciencesmolecular biology Keywords Photodynamic Therapy (PDT) Hemoglobin based nanocarriers (HOBCs) oxygen carriers cancer imaging Programme(s) H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions Main Programme H2020-EU.1.3.3. - Stimulating innovation by means of cross-fertilisation of knowledge Topic(s) MSCA-RISE-2018 - Research and Innovation Staff Exchange Call for proposal H2020-MSCA-RISE-2018 See other projects for this call Funding Scheme MSCA-RISE - Marie Skłodowska-Curie Research and Innovation Staff Exchange (RISE) Coordinator ASOCIACION CENTRO DE INVESTIGACION COOPERATIVA EN BIOMATERIALES- CIC biomaGUNE Net EU contribution € 547 400,00 Address Paseo miramon 182, parque tecnologico de san sebastian edificio empresarial c 20009 San sebastian Spain See on map Region Noreste País Vasco Gipuzkoa Activity type Research Organisations Links Contact the organisation Opens in new window Website Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Other funding € 0,00 Participants (3) Sort alphabetically Sort by Net EU contribution Expand all Collapse all CHARITE - UNIVERSITAETSMEDIZIN BERLIN Germany Net EU contribution € 239 200,00 Address Chariteplatz 1 10117 Berlin See on map Region Berlin Berlin Berlin Activity type Higher or Secondary Education Establishments Links Contact the organisation Opens in new window Website Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Other funding € 0,00 TARTU ULIKOOL Estonia Net EU contribution € 110 400,00 Address Ulikooli 18 51005 Tartu See on map Region Eesti Eesti Lõuna-Eesti Activity type Higher or Secondary Education Establishments Links Contact the organisation Opens in new window Website Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Other funding € 0,00 Surflay Nanotec GmbH Germany Net EU contribution € 23 000,00 Address Max planck strasse 3 12489 Berlin See on map SME The organization defined itself as SME (small and medium-sized enterprise) at the time the Grant Agreement was signed. Yes Region Berlin Berlin Berlin Activity type Private for-profit entities (excluding Higher or Secondary Education Establishments) Links Contact the organisation Opens in new window Website Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Other funding € 0,00 Partners (4) Sort alphabetically Sort by Net EU contribution Expand all Collapse all Partner Partner organisations contribute to the implementation of the action, but do not sign the Grant Agreement. FUNDACAO UNIVERSIDADE DE BRASILIA Brazil Net EU contribution € 0,00 Address Campus universitario darcy ribeiro faculdade de tecnologie at 05 bairro asa norte 70919 970 Brasilia df See on map Activity type Higher or Secondary Education Establishments Links Contact the organisation Opens in new window Website Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Other funding € 0,00 Partner Partner organisations contribute to the implementation of the action, but do not sign the Grant Agreement. UNIVERSIDAD NACIONAL DE GENERAL SAN MARTIN Argentina Net EU contribution € 0,00 Address Martin de irigoyen 3100 1650 San martin buenos aires See on map Activity type Higher or Secondary Education Establishments Links Contact the organisation Opens in new window Website Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Other funding € 124 384,00 Partner Partner organisations contribute to the implementation of the action, but do not sign the Grant Agreement. UNIVERSIDAD NACIONAL DEL SUR Argentina Net EU contribution € 0,00 Address Avenida colon 80 8000 Bahia blanca See on map Activity type Higher or Secondary Education Establishments Links Contact the organisation Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Other funding € 190 486,00 Partner Partner organisations contribute to the implementation of the action, but do not sign the Grant Agreement. CHULALONGKORN UNIVERSITY Thailand Net EU contribution € 0,00 Address Phyathai road 254 pathumwan 10330 Bangkok See on map Activity type Higher or Secondary Education Establishments Links Contact the organisation Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Other funding € 401 212,00
