Bacterial Enzymes and Bioprocesses for Lignin Valorisation

Project Information

B-LigZymes

Grant agreement ID: 824017

DOI

10.3030/824017

Project closed

EC signature date 13 November 2018

Start date 1 February 2019

End date 31 July 2024

Funded under

• EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions

Total cost

€ 1 025 800,00

EU contribution

€ 970 600,00

Coordinated by

UNIVERSIDADE NOVA DE LISBOA
Portugal

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Deliverables

Websites, patent fillings, videos etc. (2)

Website establishment

Web-site of the project

Social-Media accounts set-up

Facebook, Linkedin and Twitter accounts set up

Publications

Peer reviewed articles (14)

Spectroelectrochemistry for determination of the redox potential in heme enzymes: Dye-decolorizing peroxidases

Author(s): Catarina Barbosa, Carolina F. Rodrigues, Nikola Lončar, Lígia O. Martins, Smilja Todorovic, Célia M. Silveira
Published in: BBA Advances, Issue 5, 2024, Page(s) 100112, ISSN 2667-1603
Publisher: Elsevier
DOI: 10.1016/j.bbadva.2023.100112

Resonance Raman view of the active site architecture in bacterial DyP-type peroxidases

Author(s): Célia M. Silveira, Elin Moe, Marco Fraaije, Lígia O. Martins, Smilja Todorovic
Published in: RSC Advances, Issue 10/19, 2020, Page(s) 11095-11104, ISSN 2046-2069
Publisher: Royal Society of Chemistry
DOI: 10.1039/d0ra00950d

Mechanistic Insights into Glycoside 3-Oxidases Involved in C-Glycoside Metabolism in Soil Microorganisms

Author(s): André Taborda; Tomas Frazão; Miguel V. Rodrigues; Xavier Fernández-Luengo; Ferran Sancho; Maria Fátima Lucas; Carlos Frazão; M. Rita Ventura; Laura Masgrau; Patrícia T. Borges; Ligia O Martins
Published in: Crossref, Issue 4, 2023, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.21203/rs.3.rs-2662172/v1

Electrochemical Actuation of a DyP Peroxidase: A Facile Method for Drastic Improvement of the Catalytic Performance

Author(s): Magalí F. Scocozza, Francisco Vieyra, Fernando Battaglini, Ligia O. Martins, Daniel H. Murgida
Published in: ACS Catalysis, Issue 13, 2024, Page(s) 7437-7449, ISSN 2155-5435
Publisher: American Chemical Society
DOI: 10.1021/acscatal.3c01530

Loops around the Heme Pocket Have a Critical Role in the Function and Stability of BsDyP from Bacillus subtilis

Author(s): Carolina F. Rodrigues, Patrícia T. Borges, Magali F. Scocozza, Diogo Silva, André Taborda, Vânia Brissos, Carlos Frazão, Lígia O. Martins
Published in: International Journal of Molecular Sciences, Issue 22/19, 2021, Page(s) 10862, ISSN 1422-0067
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms221910862

Direct Electrochemical Generation of Catalytically Competent Oxyferryl Species of Classes I and P Dye Decolorizing Peroxidases

Author(s): Magalí F. Scocozza, Lígia O. Martins and Daniel H. Murgida
Published in: International Journal of Molecular Sciences, 2021, ISSN 1422-0067
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms222212532

Rationally Guided Improvement of NOV1 Dioxygenase for the Conversion of Lignin-Derived Isoeugenol to Vanillin

Author(s): Mario De Simone, Laura Alvigini, Lur Alonso-Cotchico, Vânia Brissos, Jonatan Caroli, Maria Fátima Lucas, Emanuele Monza, Eduardo Pinho Melo, Andrea Mattevi, and Lígia O. Martins
Published in: Biochemistry, 2022, ISSN 2155-5435
Publisher: American Chemical Society
DOI: 10.1021/acs.biochem.2c00168

Distal Mutations Shape Substrate-Binding Sites during Evolution of a Metallo-Oxidase into a Laccase

Author(s): Vânia Brissos, Patrícia T. Borges, Reyes Núñez-Franco, Maria Fátima Lucas, Carlos Frazão, Emanuele Monza, Laura Masgrau, Tiago N. Cordeiro, and Lígia O. Martins
Published in: ACS Catalysis, 2022, ISSN 2155-5435
Publisher: American Chemical Society
DOI: 10.1021/acscatal.2c00336

SERR Spectroelectrochemistry as a Guide for Rational Design of DyP-Based Bioelectronics Devices

Author(s): Lidia Zuccarello, Catarina Barbosa, Edilson Galdino, Nikola Lončar, Célia M. Silveira, Marco W. Fraaije, Smilja Todorovic
Published in: International Journal of Molecular Sciences, Issue 22, 2024, Page(s) 7998, ISSN 1422-0067
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms22157998

Immobilized dye-decolorizing peroxidase (DyP) and directed evolution variants for hydrogen peroxide biosensing

Author(s): Catarina Barbosa, Célia M. Silveira, Diogo Silva, Vânia Brissos, Peter Hildebrandt, Lígia O. Martins, Smilja Todorovic
Published in: Biosensors and Bioelectronics, Issue 153, 2020, Page(s) 112055, ISSN 0956-5663
Publisher: Pergamon Press Ltd.
DOI: 10.1016/j.bios.2020.112055

One-Pot Biocatalytic Synthesis of <i>rac</i>-Syringaresinol from a Lignin-Derived Phenol

Author(s): Yiming Guo, Laura Alvigini, Mohammad Saifuddin, Ben Ashley, Milos Trajkovic, Lur Alonso-Cotchico, Andrea Mattevi, Marco W. Fraaije
Published in: ACS Catalysis, Issue 13, 2023, Page(s) 14639-14649, ISSN 2155-5435
Publisher: American Chemical Society
DOI: 10.1021/acscatal.3c04399

Mutational and structural analysis of an ancestral fungal dye‐decolorizing peroxidase

Author(s): Ulises A. Zitare, Mohamed H. Habib, Henriette Rozeboom, Maria L. Mascotti, Smilja Todorovic, Marco W. Fraaije
Published in: The FEBS Journal, 2020, ISSN 1742-464X
Publisher: Blackwell Publishing Inc.
DOI: 10.1111/febs.15687

Flexible active-site loops fine-tune substrate specificity of hyperthermophilic metallo-oxidases

Author(s): Vânia Brissos, Patrícia T. Borges, Ferran Sancho, Maria Fátima Lucas, Carlos Frazão, Felipe Conzuelo, Lígia O. Martins
Published in: JBIC Journal of Biological Inorganic Chemistry, Issue 29, 2024, Page(s) 339-351, ISSN 1432-1327
Publisher: Springer
DOI: 10.1007/s00775-023-02040-y

ALACEN: A Holistic Herbaceous Biomass Fractionation Process Attaining a Xylose-Rich Stream for Direct Microbial Conversion to Bioplastics

Author(s): Salvador Bertran-Llorens, Wen Zhou, Martín A. Palazzolo, Dana l. Colpa, Gert-Jan W. Euverink, Janneke Krooneman, Peter J. Deuss
Published in: ACS Sustainable Chemistry &amp; Engineering, Issue 12, 2024, Page(s) 7724-7738, ISSN 2168-0485
Publisher: American Chemical Society
DOI: 10.1021/acssuschemeng.3c08414