Project description DEENESFRITPL An easy protocol for generating iPSCs Induced pluripotent stem cells (iPSCs) are a type of pluripotent stem cells that emerge from the reprogramming of somatic cells through the ectopic overexpression of defined growth factors. Many protocols for iPSC generation involve the genetic delivery of these growth factors by viral and non-viral vectors. However, most protocols are laborious and maintain cells in expensive culture media. The EU funded EASY-IPS project proposes a simpler and highly reproducible protocol for producing iPSCs using helper-dependent adenovirus vectors. Given that iPSCs are a powerful tool for modelling human disease and can be projected to serve as a regenerative cell source, the proposed protocol will help expand the applications of these cells. Show the project objective Hide the project objective Objective The discovery of induced pluripotent stem cells (iPSC) has provided a major advance in biomedical research as they are able to offer meaningful models to investigate human diseases and biology. A growing number of research laboratory are using iPSC for studying a wide variety of human diseases. iPSC have also tremendous potential for regenerative medicine because they can be virtually differentiated in any type of cell. Through the support of the ERC grant IEMTx, we built-up an in-house protocol for generation of iPSC from patients’ cell lines that is efficient and has an extremely low cost compared to other available methods with the highest clinical safety. Therefore, we can offer a superior system to produce iPSC based on a simpler, more efficient, highly reproducible, cost effective and customizable process. Moreover, HDAd have potential also for cell trans-differentiation that is the reprogramming of one somatic cell type into another cell type without passing through the pluripotent state. In summary, the goal of this proposal is to pave the way towards commercialization of our novel products for generating iPSC and trans-differentiated cell lines based on HDAd vectors as non-integrating, high-cloning capacity, inexpensive and easy to use vectors. We believe this method will become a relevant opportunity for biomedical research and might find applications in regenerative medicine as well. Fields of science medical and health sciencesmedical biotechnologycells technologiesstem cells Programme(s) H2020-EU.1.1. - EXCELLENT SCIENCE - European Research Council (ERC) Main Programme Topic(s) ERC-2018-PoC - ERC Proof of Concept Grant Call for proposal ERC-2018-PoC See other projects for this call Funding Scheme ERC-POC - Proof of Concept Grant Coordinator FONDAZIONE TELETHON ETS Net EU contribution € 150 000,00 Address Via varese 16/b 00185 Roma Italy See on map Region Centro (IT) Lazio Roma Activity type Research Organisations Links Contact the organisation Opens in new window Website Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Other funding € 0,00 Beneficiaries (1) Sort alphabetically Sort by Net EU contribution Expand all Collapse all FONDAZIONE TELETHON ETS Italy Net EU contribution € 150 000,00 Address Via varese 16/b 00185 Roma See on map Region Centro (IT) Lazio Roma Activity type Research Organisations Links Contact the organisation Opens in new window Website Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Other funding € 0,00
