An easy protocol for generating iPSCs
Induced pluripotent stem cells (iPSCs) are a type of pluripotent stem cells that emerge from the reprogramming of somatic cells through the ectopic overexpression of defined growth factors. Many protocols for iPSC generation involve the genetic delivery of these growth factors by viral and non-viral vectors. However, most protocols are laborious and maintain cells in expensive culture media. The EU funded EASY-IPS project proposes a simpler and highly reproducible protocol for producing iPSCs using helper-dependent adenovirus vectors. Given that iPSCs are a powerful tool for modelling human disease and can be projected to serve as a regenerative cell source, the proposed protocol will help expand the applications of these cells.
Funding SchemeERC-POC - Proof of Concept Grant
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