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CORDIS

Chloroplast and Mitochondria interactions for microalgal acclimation

Project description

Exploring light-dependent reactions that maximise phytoplankton photosynthesis

Photosynthesis, an important biological process on Earth, has permitted the colonisation of land and sea by plants and phytoplankton, respectively. Research is now focused on elucidating this process in phytoplankton. From an energy point of view, a microalga is a ‘vehicle’ containing mitochondria – a combustion engine using carbon to produce energy and CO2, and chloroplast – a solar panel using light to produce energy and O2. Using optogenetic approaches and cellular/subcellular imaging, the EU-funded ChloroMito project will investigate the hypothesis that the photosynthetic efficiency of oceanic microalgae is due to close coupling between their solar panel and their combustion engine. Research will shed more insight on how subcellular energy interactions optimise photosynthesis in marine phytoplankton.

Objective

Photosynthesis emerged as an energy-harvesting process at least 3.5 billion years ago, first in anoxygenic bacteria and then in oxygen-producing organisms, which led to the evolution of complex life forms with oxygen-based metabolisms (e.g. humans). Oxygenic photosynthesis produces ATP and NADPH, and the correct balance between these energy-rich molecules allows assimilation of CO2 into organic matter. Although the mechanisms of ATP/NADPH synthesis are well understood, less is known about how CO2 assimilation was optimised. This process was essential to the successful phototrophic colonisation of land (by Plantae) and the oceans (by phytoplankton). Plants optimised CO2 assimilation using chloroplast-localised ATP-generating processes to control the ATP/NADPH ratio, but the strategies developed by phytoplankton are poorly understood. However, diatoms—ecologically successful ocean organisms—are known to control this ratio by exchanging energy between plastids and mitochondria. Is this mechanism a paradigm for optimisation of photosynthesis in the ocean? The ChloroMito project aims to first decipher the mechanism(s) behind plastid-mitochondria interactions. Thanks to a novel combination of whole-cell approaches, including (opto)genetics, cellular tomography and single-cell spectroscopy, we will identify the nature of the exchanges occurring in diatoms and assess their contribution to dynamic responses to environmental stimuli (light, temperature, nutrients). We will then assess conservation of this mechanism in ecologically relevant phytoplankton taxa, test its role in supporting different lifestyles (autotrophy, mixotrophy, photosymbiosis) encountered in the ocean, and track transitions between these different lifestyles as part of an unprecedented effort to visualise ocean dynamics. Overall, the ChloroMito project will alter our understanding of ocean photosynthesis, challenging textbook concepts which are often inferred from plant-based concepts

Host institution

CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS
Net EU contribution
€ 2 419 723,25
Address
RUE MICHEL ANGE 3
75794 Paris
France

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Region
Ile-de-France Ile-de-France Paris
Activity type
Research Organisations
Links
Total cost
€ 2 498 207,00

Beneficiaries (2)