Project description
A smart way of transforming lignin into added-value bio-aromatics
Biorefineries convert biomass into products such as biopolymers, food and cosmetic ingredients, etc. As the cornerstone for the transition to a sustainable and circular bioeconomy, biorefineries rely on nature's catalysts: enzymes. However, conventional enzyme engineering methods are slow and inefficient. The EU-funded SMARTBOX project applies computational enzyme engineering methods to screen for the most promising oxidative enzyme variants leading to reduction in time and costs for the conversion of lignin into bio-aromatics such as vanillin, FDCA and polycarbonate building-blocks. This makes oxidative biocatalysis an effective approach in biorefining. For the first time ever, SMARTBOX will combine oxidative biocatalysis with reductive catalytic fractionation technology, which will allow the economically and environmentally sustainable production of added-value lignin applications.
Objective
Although they have the potential to improve the economic and environmental sustainability of biorefineries, oxidative enzymes have not experienced a complete breakthrough yet in the biobased industries. This is mainly caused by the high cost and long time associated with traditional enzyme engineering methods such as directed evolution. SMARTBOX will develop an advanced computational engineering platform specifically for oxidative enzymes, which can automatically screen for improved enzyme variants with minimal human intervention. This is achieved by implementing several innovations into current computational screening methods, most importantly machine learning, which allows to train the algorithms with experimental results. As this significantly improves computational predictability, the time and costs associated with oxidative enzyme engineering will be reduced 10-fold compared to state-of-the-art (SOTA) directed evolution methods.
Relying on the advanced engineering platform, SMARTBOX will develop the one-enzyme conversion of HMF into FDCA and intermediates, and the one-enzyme conversion of lignin monomers into a potential biobased building block for polycarbonates and vanillin. By adopting a 1-enzyme FDCA production process, the associated production costs and carbon footprint are expected to decrease significantly compared to SOTA chemical oxidation methods. The unique feature of SMARTBOX is that reductive catalytic fractionation (RCF) will be used to selectively produce specific lignin monomers from biomass in near theoretical yields. The structural similarity of the resulting monomers with the SMARTBOX building blocks allows the development of high-yielding processes with only one enzyme. Due to the smart combination between oxidative biocatalysis and RCF, the production of bio-aromatics will proceed with higher yields than the state of the art.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
- natural scienceschemical scienceselectrochemistryelectrolysis
- natural scienceschemical sciencescatalysisbiocatalysis
- natural sciencescomputer and information sciencesartificial intelligencemachine learning
- natural sciencesbiological sciencesbiochemistrybiomoleculesproteinsenzymes
- agricultural sciencesagricultural biotechnologybiomass
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RIA - Research and Innovation actionCoordinator
9042 Desteldonk Gent
Belgium
The organization defined itself as SME (small and medium-sized enterprise) at the time the Grant Agreement was signed.