Periodic Reporting for period 1 - COMPLIMB (A computational tool to elucidate the mechanobiological regulation of limb development)
Reporting period: 2019-05-06 to 2021-05-05
Understanding the role of mechanical stimuli in joint formation can help identify when and how pathological conditions arise that result in malformations, and potentially provide clues on how to avoid or correct joint deformities. Ultimately, a better understanding of joint formation could inform therapies to correct joint deformities, as well as contribute to the development of preventive strategies for congenital limb defects.
The main goal of the CompLimb project is to determine the role of mechanical stimuli and mechanosensitive growth regulators driving joint formation in vertebrates. To achieve this goal, we use a combined experimental and computational approach. Limb formation has been widely studied in axolotl salamanders (Ambystoma mexicanum), as they regrow limbs throughout their life. Experiments on regenerating axolotl limbs provide information on how altering the cell’s ability to sense and respond to mechanical stimuli affects joint shape as well as the location and timing of molecular expression critical to joint formation. Predictive computational models informed by the experimental findings allow us to explore potential physical mechanisms of normal and pathological joint formation.
In parallel, we developed a finite element model of growth at tissue level to study how limb motion might regulate humerus morphology during joint morphogenesis. In this mathematical model of the limb, we assumed that pressure promoted growth. This pressure changes over a flexion-extension cycle of the elbow. We modelled the geometry and loading conditions of a generic axolotl humerus based on experimental data, calculated pressure during elbow flexion, and grew the model in proportion to the pressure. The computational predictions of the shape of the control and mechanosensitivity-impaired cases matched the experimental findings. Our results suggest that pressure may be an important stimulus in regulating local tissue growth during joint formation.
Our poroelastic computational model incorporates, for the first time, the effect of dynamic loading on local tissue growth. Previous models of joint morphogenesis used elastic materials, which cannot account for the fluid flow and, hence, the dynamic pressure maps that change over a loading cycle. These pressure maps are the ones dictating growth in our model. In addition, for the first time, we have developed a joint morphogenesis model informed by and validated with experimental data.
We will now start to analyse the results of the latest experiments in which we use a new technique to image the expression of morphogenetic biomolecules in healthy regenerating axolotl forelimbs around the time that the joint appears (cavitation). Studies to date have been limited because techniques to quantify molecular expression loose spatial patterning, while methods that allow visualizing their expression in space have been, thus far, exclusively in 2D sections and non-quantifiable. Our new technique will allow mapping in 3D the location of these biomarkers. We will use the experimental results to develop in parallel a new computational model to predict joint cavitation. Together, they will provide a better understanding of the exact biochemical conditions required for healthy limb formation and, more specifically, how the biophysical environment influences these molecular components responsible for growth.
The findings derived from the CompLimb project will contribute to a better understanding of vertebrate joint formation. This knowledge might ultimately have an impact on the development of therapies to treat joint deformities or approaches to prevent congenital malformations.