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Integrated on-farm Aquaponics systems for co-production of fish, halophyte vegetables, bioactive compounds, and bioenergy

Deliverables

Assessment of immune status and inflammatory response in fish and shrimp fed the different halophyte-based diets

This document will report the methodology and results of the analysis carried out to asses immune condition and inflammatory response of fish and shrimp fed with halophyte supplemented diets.

First batch of Practice Abstracts

"The resulting innovative knowledge and easy accessible end-user material from this project will feed into the EIP-AGRI (The agricultural European Innovation Partnership) website for broad dissemination. The end-user material to be produced contains a substantial number of summaries for practitioners in the EIP common format (""practice abstracts""), including the characteristics of the project (e.g. contact details of partners, etc). A full package of practice abstracts will be produced by the project, containing all the outcomes/recommendations which are ready for practice.A practice abstract is a short summary of around 1000-1500 characters (word count – no spaces; see below) which describes the main information/recommendation/practice that can serve the end-users in their daily practice. Guidance and templates for these practice abstracts are available on the EIP-AGRI web site: http://ec.europa.eu/eip/agriculture/en/content/eip-agri-common-format . A number of practice abstracts is foreseen for the project. Estimated 25 Practice Abstracts are expected to be delivered in the first batch."

Final report on the scale-up of the transformation of lignin into pelletized biochar

Must inlcude description of the update compared to the draft report.

Draft report on optimized co-digestion process of residual fiber and juice residues

This document will report the methodology and results of the codigestion experiments on the residual fiber and juice residues The optimal ratio of the two fractions and loading of the codigestion process will be determined in batch tests Then the optimized codigestion process will be tested in semicontinuous labscale reactors optimal operation conditions like organic loading rate and hydraulic retention time of the biogas reactor will be reported

Report/paper on optimisation of simple protein precipitation by acidification

This document will report the methodology and results of simple protein extraction from the green juice by chemical and microbial acidification Protein characterisation yield stability and feasibility will be reported

Report on the optimum absorbent biochar and datasheet

This document will report the methodology and results of the lignin conversion to carbonized material by pyrolysis process The most promising preparation will be selected on the basis of resource efficiency adaptability to industrial manufacturing and expected retail price of the final product

Report/paper on hemicellulose sugars extraction from extractives-free green fibres

This document will report the methodology and results of hemicellulose sugar extraction from the green juice and fibres after removal of lipids and proteins. The hemicellulose sugars feasibility for use in XOS and biogas (WP9) will be reported.

Draft report on the scale-up of the transformation of lignin into pelletized biochar

This document will report the methodology and results of the pilot scale pyrolysis of the organosolv lignin into pelletized biochar Finetuning will be performed using 011 kg batch Pilot equipment for finetuning of activation parameters guided by yieldperformance tradeoff

Paper on stabilisation of HCA by transesterification

This deliverable will be a journal paper describing the methods and results on stabilisation of HCAs using transesterification. This will include optimal reaction conditions such as solvent composition, substrate concentration, enzyme load, time, pH and temperature.

Final report on optimized pilot scale extraction

must include description of updates compared to draft.

Handbook for lessons and best practices in implementing halophyte fractions and compounds in cosmetic, food, and feed products

This document will be a handbook on for lessons and best practices in implementing halophyte fractions and compounds in cosmetic, food, and feed products. AQUA-COMBINE will assess the procedures and requirements for the application of the developed halophyte extracts and compounds as a food ingredient according to EC regulation No. 234/2011 or any novel food regulation. To ensure future marketability of the new feed/food ingredient, desk research and close collaboration with consumer panels will clarify consumer perception with regard to adding the compounds in cosmetics and foods. Communication to the public presenting lessons and best practices learnt during the project in terms of halophyte products effect and bioactivity will be developed and given to the public, effect targeting the long-term contribution of AQUA-COMBINE to the formation of common EU policies on cosmetics, feed and food processing.

Report on the most optimal configuration for lipid and protein extraction with proposed a scale-up scheme.

This document will report the computer aided process engineering tools used to support the extraction of lipids and protein from the green juice in solvent selection and solvent recovery Beside technical feasibility sustainability aspects as well as economic aspects will be reported in close collaboration with WP10

Evaluation of the anti-genotoxic properties, alterations on DNA-damage repair system, and of enzymatic and non-enzymatic antioxidant responses

This document will describe the methodology and results of the evaluation of DNA integrity, DNA-damage repair and anti-oxidant defences of fish and shrimp fed with halophyte-supplemented diets.

Fish and shrimp growth performance, feed utilisation and digestibility of nutrients

This document will report the methodology and results of the Zootechnical trials. To evaluate the effects of halophytes containing diets in seabass and shrimp zootechnical performance will be performed and reported. This will include growth and digestibility results.

Assessment of phytochemical profiles in fish and shrimp tissues collected from Task 7.2

This document will report the methodology and results of the phytochemical profiling in key tissues of fish and shrimp fed with halophyte supplemented diets included data interlinking

Report/papers on quantification of HCAs in optimised extracts of straw from Salicornia europaea, and Crithmum maritimum

This document will report the methodologies and results of the detailed chemical composition and quantification of HCAs in optimised extracts of straw from Salicornia europaea, and Crithmum maritimum.

Report/papers on chemical composition of optimised botanical extracts of green juice and extraction juice residue from Salicornia europaea, Crithmum maritimum and Aster tripolium

This document will report the methodologies and results of the detailed chemical composition of botanical extracts of green juice and extraction juice residue from Salicornia europaea Crithmum maritimum and Aster tripolium

Project collaborative platform

Creation of project website and intranet for the consortium.

Project website

A public website will be created for dissemination of project, events and public project results.

Publications

Exploring the Antioxidant and Genoprotective Potential of Salicornia ramosissima Incorporation in the Diet of the European Seabass (Dicentrarchus labrax)

Author(s): Marçal, R., Sousa, P., Marques, A., Pereira, V., Guilherme, S., Barreto, A., Costas, B., Rocha, R., Pacheco, M.
Published in: MDPI/Animals, Issue Animals 2024, 14(1), 93, 2023, ISSN 2076-2615
Publisher: EISSN 2076-2615
DOI: 10.3390/ani14010093

Extraction Methods of Value-Added Compounds from Biomasses

Author(s): Taşçı, T.Ç., Fasse, S., Raval, S., Thomsen, M.H., Gottschalk, A.
Published in: 30th EUBCE 2022, 2022, ISSN 2282-5819
Publisher: EUBCE
DOI: 10.5071/30theubce2022-3cv.6.26

Bioactive Extracts from Salicornia ramosissima J. Woods Biorefinery as a Source of Ingredients for High-Value Industries

Author(s): Laura Sini Sofia Hulkko; Rui Miranda Rocha; Riccardo Trentin; Malthe Fredsgaard; Tanmay Chaturvedi; Luísa Custódio; Mette Hedegaard Thomsen
Published in: Hulkko , L S S , Rocha , R M , Trentin , R , Fredsgaard , M , Chaturvedi , T , Custódio , L & Thomsen , M H 2023 , ' Bioactive Extracts from Salicornia ramosissima J. Woods Biorefinery as a Source of Ingredients for High-Value Industries ' , Plants , vol. 12 , no. 6 , 1251 . https://doi.org/10.3390/plants12061251, Issue 10, 2023, ISSN 2223-7747
Publisher: MDPI/Plants
DOI: 10.3390/plants12061251

Harnessing the Value of Tripolium pannonicum and Crithmum maritimum Halophyte Biomass through Integrated Green Biorefinery

Author(s): Laura Sini Sofia Hulkko; Tanmay Chaturvedi; Luísa Custódio; Mette Hedegaard Thomsen
Published in: Marine Drugs; Volume 21; Issue 7; Pages: 380, Issue 4, 2023, ISSN 1660-3397
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/md21070380

Halophyte Plants and Their Residues as Feedstock for Biogas Production—Chances and Challenges

Author(s): Ariel E. Turcios, Aadila Cayenne, Hinrich Uellendahl, Jutta Papenbrock
Published in: Applied Sciences, Issue 11/6, 2021, Page(s) 2746, ISSN 2076-3417
Publisher: MPDI
DOI: 10.3390/app11062746

Salicornia ramosissima Biomass as a Partial Replacement of Wheat Meal in Diets for Juvenile European Seabass (Dicentrarchus labrax)

Author(s): André Barreto; Ana Couto; Daniel Jerónimo; Adriana Laranjeira; Bruna Silva; Catarina Nunes; Ana C. S. Veríssimo; Diana C. G. A. Pinto; Jorge Dias; Mário Pacheco; Benjamin Costas; Rui J. M. Rocha
Published in: Animals, Vol 14, Iss 4, p 614 (2024), Issue 4, 2024, ISSN 2076-2615
Publisher: MDPI
DOI: 10.3390/ani14040614

Isolation of Polyphenols from Aqueous Extract of the Halophyte Salicornia ramosissima

Author(s): Malthe Fredsgaard; Job Tchoumtchoua; Stephan Kohnen; Tanmay Chaturvedi; Mette Hedegaard Thomsen
Published in: Molecules, Vol 29, Iss 1, p 220 (2023), Issue 3, 2023, ISSN 1420-3049
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/molecules29010220

Extraction, Isolation, and Purification of Value-Added Chemicals from Lignocellulosic Biomass

Author(s): Tanmay Chaturvedi; Laura Sini Sofia Hulkko; Malthe Fredsgaard; Mette Hedegaard Thomsen
Published in: Chaturvedi , T , Hulkko , L S S , Fredsgaard , M & Thomsen , M H 2022 , ' Extraction, Isolation, and Purification of Value-Added Chemicals from Lignocellulosic Biomass ' , Processes , vol. 10 , no. 9 , 1752 . https://doi.org/10.3390/pr10091752, Issue 2, 2022, ISSN 2227-9717
Publisher: MDPI/Processes
DOI: 10.3390/pr10091752

Compositional Changes in Hydroponically Cultivated Salicornia europaea at Different Growth Stages

Author(s): Ariel E. Turcios; Lukas Braem; Camille Jonard; Tom Lemans; Iwona Cybulska; Jutta Papenbrock
Published in: Plants, Vol 12, Iss 13, p 2472 (2023), Issue 5, 2023, ISSN 2223-7747
Publisher: MDPI/Plandts
DOI: 10.3390/plants12132472

Pharmacological Insights into Halophyte Bioactive Extract Action on Anti-Inflammatory, Pain Relief and Antibiotics-Type Mechanisms

Author(s): Rocco Giordano, Zeinab Saii, Malthe Fredsgaard, Laura Sini Sofia Hulkko, Thomas Bouet Guldbæk Poulsen, Mikkel Eggert Thomsen, Nanna Henneberg, Silvana Maria Zucolotto, Lars Arendt-Nielsen, Jutta Papenbrock, Mette Hedegaard Thomsen, Allan Stensballe
Published in: Molecules, Issue 26/11, 2021, Page(s) 3140, ISSN 1420-3049
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/molecules26113140

Halophytes as Feedstock for Biogas Production: Composition Analysis and Biomethane Potential of Salicornia spp. Plant Material from Hydroponic and Seawater Irrigation Systems

Author(s): Aadila Cayenne, Ariel E. Turcios, Mette Hedegaard Thomsen, Rui Miranda Rocha, Jutta Papenbrock, and Hinrich Uellendahl
Published in: MDPI/Fermentation, 2022, ISSN 2311-5637
Publisher: MDPI/Fermentation
DOI: 10.3390/fermentation8040189

Enhancing the Methane Yield of Salicornia spp. via Organosolv Fractionation as Part of a Halophyte Biorefinery Concept

Author(s): Aadila Cayenne; Maxwel Monção; Leonidas Matsakas; Ulrika Rova; Paul Christakopoulos; Mette H. Thomsen; Hinrich Uellendahl
Published in: Crossref, Issue 2, 2024, ISSN 1996-1073
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/en17051074

Review on Environmental Sustainability Assessment Methodologies in a Perspective of Halophyte-Based Bio-Refinery

Author(s): Raval, S., Taşçı, T.Ç., Fasse, S., Thomsen, M.H., Gottschalk, A.
Published in: European Biomass Conference and Exhibition Proceedings ISSN 2282-5819, Issue 30th EUBCE 2022, 2022, Page(s) 361 - 365, ISSN 2282-5819
Publisher: EUBCE
DOI: 10.5071/30theubce2022-2av.3.5

A novel biorefinery concept based on marginally used halophyte biomass

Author(s): Maxwel Monção; Petter Paulsen Thoresen; Tobias Wretborn; Heiko Lange; Ulrika Rova; Paul Christakopoulos; Leonidas Matsakas
Published in: Crossref, Issue 5, 2023, ISSN 2398-4902
Publisher: Sustainable Energy & Fuels
DOI: 10.1039/d3se00458a

<i>Salicornia dolichostachya</i> organosolv fractionation: towards establishing a halophyte biorefinery

Author(s): Maxwel Monção; Tobias Wretborn; Ulrika Rova; Leonidas Matsakas; Paul Christakopoulos
Published in: Crossref, Issue 3, 2022, ISSN 2046-2069
Publisher: Royal Society of Chemistry
DOI: 10.1039/d2ra04432c

Effects of Salicornia-Based Skin Cream Application on Healthy Humans’ Experimental Model of Pain and Itching

Author(s): Rocco Giordano, Giulia Erica Aliotta, Anja Stokholm Johannesen, Dina Voetmann-Jensen, Frederikke Hillebrand Laustsen, Lasse Allermann Andersen, Aman Rezai, Malthe Fredsgaard, Silvia Lo Vecchio, Lars Arendt-Nielsen, Mette Hedegaard Thomsen, Allan Stensballe
Published in: MDPI/Pharmacuticals, 2022, ISSN 1424-8247
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ph15020150

Extraction and Quantification of Chlorophylls, Carotenoids, Phenolic Compounds, and Vitamins from Halophyte Biomasses

Author(s): Laura S. S. Hulkko, Tanmay Chaturvedi, Mette Hedegaard Thomsen
Published in: Applied Sciences, 2022, ISSN 2076-3417
Publisher: MDPI/Applied Sciences
DOI: 10.3390/app12020840

Halophytes Used in an Integrated Biorefinery with the Extraction of Bioactive Compounds

Author(s): Fasse, S., Taşçı, T.Ç., Raval, S., Thomsen, M.H., Gottschalk, A.
Published in: 30th EUBCE 2022, 2022, ISSN 2282-5819
Publisher: EUBCE
DOI: 10.5071/30theubce2022-3bo.6.3

Phenolic Compounds in Salicornia spp. and Their Potential Therapeutic Effects on H1N1, HBV, HCV, and HIV: A Review

Author(s): Malthe Fredsgaard; Samba Evelyne Kabemba Kaniki; Io Antonopoulou; Tanmay Chaturvedi; Mette Hedegaard Thomsen
Published in: Fredsgaard , M , Kaniki , S E K , Antonopoulou , I , Chaturvedi , T & Thomsen , M H 2023 , ' Phenolic Compounds in Salicornia spp. and Their Potential Therapeutic Effects on H1N1, HBV, HCV, and HIV : A Review ' , Molecules , vol. 28 , no. 14 , 5312 . https://doi.org/10.3390/molecules28145312, Issue 10, 2023, ISSN 1420-3049
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/molecules28145312

Modeling, simulation and techno-economic analysis of an integrated biorefinery based on halophytes

Author(s): Tutku Taşçı Çilak; Sanketkumar Raval; Sylvia Fasse; Mette H. Thomsen; Axel Gottschalk
Published in: Computer Aided Chemical Engineering ISBN: 9780443152740, Issue 6, 2023, ISSN 1570-7946
Publisher: Elsevier
DOI: 10.1016/b978-0-443-15274-0.50392-9

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