Objective
The present project aims at the preparation of synthetic nanoreceptors exhibiting glycan epitope recognition in glycoproteins, identified to play a critical role in cell biology and diseases. Such nanoreceptors, behaving like antibodies, may cope with the current limitations in the essential step of enrichment of specific glycan subclasses, within studies of the glycoproteome. The preparation of the receptors will proceed through solid-phase synthesis of imprinted polymer nanoparticles, with the potential to deliver entities with size, specificity and solubility characteristics comparable to antibodies. The solid-phase synthesis of NanoMIP will require the immobilization of important monosaccharides (N-acetylglucosamine, N-acetlgalactosamine, mannose), widely found in glycoproteins, onto supporting beads. Several synthetic routes will be studied for the immobilization onto silica or plastic beads . In a reactor, built as a controlled temperature glass column filled with the beads containing immobilized monosaccharide, criteriously selected polymerization or sol-gel mixtures will generate the nanoMIPs, with subsequent selection of the highest affinity particles. The optimum combinations of solid-phase/imprinting protocol will be screened from a myriad of nanoMIPs prepared in different conditions. The performance of the nanoMIPs will be ascertained by setting a competitive enrichment protocol consisting of the incubation with a mixture of N-acetylglucosamine-, N-acetylgalactosamine- and mannose-containing glyproteins and a non-glycoprotein. Sorption isotherm data will be used to derive important binding features such as capacity, binding affinity and selectivity. Patentable robust solid-phases and imprinting protocols, either in standalone or combined manner, are planned outputs of the project, which may, in the near future, be incorporated into existing automated solid-phase synthesis instrumentation, for increased throughput and batch reproducibility.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences cell biology
- natural sciences chemical sciences polymer sciences
- engineering and technology materials engineering
- natural sciences biological sciences biochemistry biomolecules carbohydrates
- engineering and technology nanotechnology nano-materials
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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H2020-EU.4. - SPREADING EXCELLENCE AND WIDENING PARTICIPATION
MAIN PROGRAMME
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Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
MSCA-IF-EF-ST - Standard EF
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Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) H2020-WF-2018-2020
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Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
4099-002 PORTO
Portugal
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.