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DNA-guided self-organized active materials

Project description

Materials science: lessons from embryonic development

Morphogenesis is the biological process responsible for producing the complex shapes of organisms from the blastocyst. It is a multistep process orchestrated by biomolecules known as morphogens. Inspired by morphogenesis, the EU-funded DNAGAM project is working towards the first-ever synthetic biocompatible material that can self-organise in a programmable and autonomous manner. The biomaterial incorporates a DNA-based chemical network capable of generating single-stranded DNA morphogens in a unique yet spatially predictable manner. The DNA morphogens will guide kinesin motor proteins and microtubules to form morphological structures within hydrogels. The project's results will not only provide invaluable insight into how morphogen patterns drive material self-organisation, but also expand materials science applications in soft robotics and biological environments.

Objective

Programming the autonomous and multiscale structuring of shapeless synthetic soft matter is unknown and conceptually challenging. In stark contrast, a living embryo is highly ordered at all levels – from cells to the entire organism. The ordering is a multistep process, starting from the patterning of biomolecules (morphogens) which later instruct autonomous shape transformations (morphogenesis). Inspired by these natural physicochemical processes, we aim at the preparation of a first-ever synthetic biocompatible material which can be self-organized in a programmable and autonomous manner. The programming will be achieved by an out-of-equilibrium DNA-based chemical network which predictably generates single-stranded DNA morphogens. Combined with diffusion, the concentrations of the morphogen can be patterned with a unique spatiotemporal precision, including travelling waves and stable fronts, which were pioneered by the host group. The autonomy of morphological structuring will be accomplished by linking the mechanical activity of active gels, composed of DNA-kinesins and microtubules, to the presence of the DNA morphogen. Latter will act as a cross-linker creating the clusters of kinesins and thus guiding the self-organization of the soft material by the collective action of nanoscale kinesin motor proteins which exert force on microtubules. Apart from the preparation of a first biocompatible man-made morphogenetic material, we will learn how the self-organization of active gels is dependent on morphogens’ patterns. This knowledge is indispensable for the advanced programming of the precise macroscale shapes at the molecular level of chemical networks, which are diverse and modular. With further developments, our methodology could lead to so far elusive self-fabricated, force-exerting synthetic soft matter with the potential of integration in soft robotics and biological environments.

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Topic(s)

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MSCA-IF-EF-CAR - CAR – Career Restart panel

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Call for proposal

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(opens in new window) H2020-MSCA-IF-2019

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Coordinator

THE CHANCELLOR MASTERS AND SCHOLARS OF THE UNIVERSITY OF CAMBRIDGE
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 73 765,44
Address
TRINITY LANE THE OLD SCHOOLS
CB2 1TN CAMBRIDGE
United Kingdom

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Region
East of England East Anglia Cambridgeshire CC
Activity type
Higher or Secondary Education Establishments
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

€ 73 766,09

Participants (1)

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