Valuation of the global olive oil market is expected to reach USD ~11 billion by 2025, with Extra Virgin Olive Oil (EVOO) segment expected to reach a market value of USD ~2.5 billion by the end of 2024. EVOO due to its increasing popularity as a health-food, and its application in cosmetics/pharmaceuticals has become an ideal fraud-target, with the most common adulteration practices (admixtures/substitution) targeting its quality, purity and origin. This issue is yet to be fully addressed, since it jeopardizes sustainability, deceiving consumers, reducing valuation and in parallel undermining safety, promoting unfair commercial practices. Nowadays, producers, manufacturers, B2B and retailers of EVOO demand accurate solutions to protect quality and health claims. AUTHEVOO addresses an end-to-end DNA-based system of EVOO authenticity and traceability, delivering complete EVOO supply chain transparency from field to store. Its technology displays a significant positive outcome for the environment and the society, embracing agrobiodiversity by adding-value to local varieties, supporting local producers by valorizing their produce, and overall enhancing sustainability. The above aligns with the Green Deal of the EU (Farm-to-Fork) supporting strategies aiming for balance between nature, food and biodiversity, and consumers’ health and well-being. Therefore, the necessity for verifiable and immutable transparency, traceability and authenticity in the olive oil industry becomes an imperative action.