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New emitting proteins and all-bio matrices for Bio-WLEDs.

Project description

New fluorescent proteins and bio-matrices for eco-friendly illumination systems

White inorganic light-emitting diodes (WLEDs) provide excellent efficiency and stability but rely on rare earth chemical elements. Bio-hybrid WLEDs (Bio-WHLEDs) represent a sustainable and cost-effective alternative using fluorescent proteins (FPs) embedded into a synthetic polymer matrix. Currently, Bio-WHLEDs are limited to low-power applications as the continued excitation of FPs leads to denaturation. The EU-funded NMPLED project aims to design and develop improved matrices and proteins for the new generation of all bio-based colour down-converting coatings. Researchers propose to encapsulate the FPs into natural source biopolymers-based packaging matrices and replace the GFP-based proteins with a new class of dedicated FPs to increase the overall photostability of the FPs.

Objective

The development of eco-friendly sun-like illumination systems is a priority in Europe. Current solutions, White Inorganic Light-Emitting Diodes (WLEDs), feature excellent efficiency and stability values but rely on rare earth and their production involves an increasing economic and ecological cost. Bio-Hybrid WLEDs (Bio-WHLEDs) represent an environmentally sustainable and cost-effective alternative. They use Fluorescent Proteins (FPs) embedded into a synthetic polymer matrix and act as the color down-converting coating. Bio-WHLEDs are currently limited to low power applications because the continued excitation of FPs promotes its denaturation. This is due to the synthetic nature of the polymeric packaging and of the type of FPs used (generally variants of Green Fluorescent Protein (GFP)). Despite the well-known brightness of such proteins, they have evolved as genetic reporters and not as stable photo-converting materials. In this project, we aim to design, produce and exploit improved matrixes and proteins for the new generation of all bio-based color down-converting coating. To tackle the first limitation of current Bio-WHLEDs, we propose the encapsulation of FPs into marine polysaccharide-based packaging matrices such as bio-doped alginate/ ulvan microparticles. Since the interior of these particles offers a crowded environment more similar to a natural protein surrounding, they are supposed to reduce the thermal stress and increase the photostability of the FPs. To tackle the second limitation, we propose the replacement of GFP-based proteins by Phycobiliproteins (PBPs). PBPs have evolved in nature as light-harvesting antennas in cyanobacteria, being resistant to extreme conditions (high temperature, constant illumination, high concentrations), but emit light only above 550 nm. To give a new twist in originality we propose the use of error-prone PCR and selective evolution to modify PBPs characteristics to obtain red-, green- and orange- emissions

Fields of science (EuroSciVoc)

Keywords

Programme(s)

Topic(s)

Funding Scheme

MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF)

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Call for proposal

(opens in new window) H2020-MSCA-IF-2019

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Coordinator

FUNDACION IMDEA MATERIALES
Net EU contribution
€ 172 932,48
Address
CALLE ERIC KANDEL 2 PARQUE CIENTIFICO Y TECNOLOGICO TECNOGETAFE
28906 Getafe
Spain
Region
Comunidad de Madrid Comunidad de Madrid Madrid
Activity type
Research Organisations
Links
Total cost
€ 172 932,48