Project description
High throughput second harmonic generation to probe biological surfaces
Biological surfaces are essential for life; they provide a substrate for chemical signalling, allow the active and passive transport of substances and contribute to the specialised compartmentalisation in eukaryotic cells. The EU-funded H2O-SurfaceProbe project aims to enable the probing of biological surfaces using a second harmonic (SH) response methodology. A new high throughput SH setup will be introduced in combination with ~3 orders of magnitude enhancement in the SH response to enable probing at biologically relevant (~100 mM) salt concentrations. Using this new high throughput setup, two different biological membrane systems and supported reactions will be elucidated: biomimetic lipid droplet organelle and liposomes.
Objective
Biological surfaces are essential for life. Nature employs them as a hub for chemical signalling, active and passive transport of substances, and specialized compartmentation in the building block of life, eukaryotic cell. As such it has been in the main focus of chemist, physicist, and biologist to probe these surfaces with a wide variety of different techniques; from NMR to STORM. This, in fact, is not an easy task since obtaining selective information only from surfaces generally requires to introduce some reporting molecules (chromophore). However, the response of a dye molecule is not always, report on the changes on the biological surface. In this IF proposal, the aim is to enable the probing of biological surfaces, free-floating model membranes, label-free, and non-invasive from the response of interfacial water molecules with second harmonic (SH) response. First, a new high-throughput SH setup will be introduced. The proposed ~3 orders of magnitude enhancement in the SH response will enable to probe at biology relevant (~100 mM) salt concentrations. A big accomplishment for the SH research field. Second, by using this new high throughput setup, two different biological membrane systems and reactions occurring there at will be elucidated, biomimetic lipid droplet organelle, and liposomes, two free-floating membrane model system. Due to its novel aspects, a significant impact is expected from the deliverables of this proposal not only for the scientific field, but also possible intersectoral collaboration prospects as well as opening opportunities for the researcher to achieve a permanent position.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions
MAIN PROGRAMME
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H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility
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Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF)
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Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) H2020-MSCA-IF-2019
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Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
06800 BILKENT ANKARA
Türkiye
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.