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Volumetric light-driven bioprinting capturing complex physiological shape, size and function in artificial tissues and organoids

Project description

A novel 3D printing technique for reproducing parts of the human body

Lab-grown tissues and organoids promise to revolutionise medicine and biology, tackling transplant shortage and introducing accurate in vitro models of human physiology as an alternative to animal experimentation. The functionality of living organs is intimately linked to their complex architecture. Advances in key technologies capturing this shape-function relationship in vitro can help realise the long-sought goal of real tissue engineering. The EU-funded VOLUME-BIO project plans to develop a new bioprinting technique to precisely create engineered tissues and organoids exhibiting physiological functions. Cell-laden hydrogels will be sculpted into tissue analogues within seconds, upon exposure to a light field.

Objective

Lab-made artificial tissues and organoids promise to revolutionize medicine, tackling transplant shortage, and to innovate biological and pharmaceutical research, introducing accurate in vitro models of human physiology, as potential alternatives to animal experimentation. The functionality of living organs is intimately linked to their complex architecture, from the physicochemical properties of extracellular microenvironment, to tissue-level scale, where multiple cell populations interact in a precisely orchestrated spatial distribution. Advances in key technologies capturing this shape-function relationship in vitro can bring the long-sought goal of real tissue engineering within reach.

In VOLUME-BIO I will develop a novel multi-material volumetric bioprinting technology for the precise generation of engineered tissues and organoids exhibiting physiological functions. Inspired by optical tomography, cell-laden hydrogels are sculpted into tissue analogues within seconds, upon exposure to bio-friendly 3D visible light fields. Tuneable light patterns control the local distribution of cells and, through orthogonal photo-chemical reactions, of key factors that guide stem cell fate, namely stiffness of the extracellular matrix and morphogenetic biochemical cues. The unprecedented ability to tune independently such parameters will also permit to build 3D platforms to study how architectural complexity impacts organoid maturation. This will provide a new tool to address the so far unanswered question of how much an engineered tissue needs to mimic Nature’s template to achieve physiological functionality.

Bringing together my expertise in engineering, bioprinting, materials design and stem cell biology, I will first test the potential and versatility of this novel volumetric technology by building from anatomical patient-specific images functional and centimetre-scale vascularized bone and bone marrow organoid supporting physiological-like hematopoiesis.

Host institution

UNIVERSITAIR MEDISCH CENTRUM UTRECHT
Net EU contribution
€ 1 554 634,14
Address
HEIDELBERGLAAN 100
3584 CX Utrecht
Netherlands

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Region
West-Nederland Utrecht Utrecht
Activity type
Higher or Secondary Education Establishments
Links
Total cost
€ 1 554 634,14

Beneficiaries (2)