Novel approach to study allosteric regulation in CRISPR-Cas9 nucleoprotein complex
Allosteric regulation in biochemistry is the regulation of an enzyme by binding an effector molecule at a site other than the active site of the enzyme. Effectors that enhance the protein's activity are called 'allosteric activators', in contrast to 'allosteric inhibitors' that decrease the protein's activity. CRISPR-Cas9 is a large nucleoprotein complex, widely employed as a genome editing tool. Its intricate allosteric signaling involves the multi-domain Cas9 protein and its associated nucleic acids, controlling the system's function and specificity. The EU-funded Allosteric-CRISPR project will investigate the allosteric regulation in CRISPR-Cas9 by introducing a novel synergistic approach as an emerging powerful tool to investigate allostery. This approach combines state-of-the-art theoretical methods with network models derived from graph theory.
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