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Synthetic Biology of mRNA

Project description

Exploiting non-standard forms of mRNA

Messenger RNA (mRNA) is responsible for translating genetic information into proteins, which are the key drivers of biological processes. However, so far we are only scratching the surface when it comes to exploiting the application potential of these molecules. Scientists of the EU-funded NEWmRNA project argue that a new approach to studying and producing standard and non-standard forms of mRNA is needed given its emerging role in vaccination, chemistry, diagnostics and pest control. Therefore, they will follow a synthetic biology approach for studying and manufacturing mRNA with biological value.

Objective

mRNA is a major actor in the expression of cellular information, but we can only wield it in the very narrow boundaries imposed upon us by the transcriptional machinery and our lack of knowledge of the chemical scope of mRNA translation inhuman cells. Given the diverse role of mRNA in major life science applications from vaccination via gene therapy, chemistry, and diagnostics to pest control in agriculture, we argue that we are missing a huge opportunity to improve the outcome of medical therapies, broaden chemical catalysis, advance medical diagnostics, and reduce the human footprint in agriculture. With a focus on medical applications, NEWmRNA proposes to follow a synthetic biology approach to redraw the chemical map of the translation of mRNAs in human cells and at the same time to transform biological mass production of mRNAs. In the course of the project, we will implement a novel cellular assay to rapidly assess the impact of non-canonical forms of mRNA on translation and cellular signaling, re-engineer enzymatic mRNA synthesis via T7 RNA polymerase, introduce the concept of cloaked bioproduction in bacteria – the production of protected forms of instable molecules, coupled to enzymatic recovery post production – and introduce crucial steps for industrial-scale production of mRNA.

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Keywords

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Programme(s)

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Topic(s)

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Funding Scheme

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RIA - Research and Innovation action

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Call for proposal

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(opens in new window) H2020-FETOPEN-2018-2020

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Coordinator

EIDGENOESSISCHE TECHNISCHE HOCHSCHULE ZUERICH
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 484 177,50
Address
Raemistrasse 101
8092 Zuerich
Switzerland

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Region
Schweiz/Suisse/Svizzera Zürich Zürich
Activity type
Higher or Secondary Education Establishments
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

€ 484 177,50

Participants (5)

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