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One-step switchable fluorescent probe assay for direct virus detection.

Project description

Screening for SARS-CoV-2 becomes faster, cheaper and more accurate

Presently, SARS-CoV-2 tests are performed using the polymerase chain reaction (PCR) method. PCR tests require advanced laboratory equipment for the various steps, and turnaround times are slow. The EU-funded 1-SWITCH project is developing a new single-step switchable fluorescent probe assay for SARS-CoV-2 detection. The technology involves affordable reagents, and the sensitivity of the test is projected to be superior to COVID-19 antigen tests while being comparable to PCR tests. The one-step, one-tube test will detect the virus within minutes at the point of care, providing a major breakthrough in clinical COVID-19 diagnostics.

Objective

Since March 2020, the world is facing the Covid-19 pandemic. As of September 2020, over 900 000 people have died of the disease, caused by the SARS-CoV-2 virus. The WHO recommendations in order to limit the spread of the virus are to “test, trace and isolate”. To be successful, this strategy requires fast and massive testing. Today, tests are performed using PCR, a resource-intensive process requiring extraction of the viral RNA before nucleic acid amplification. PCR tests are slow (several hours) and require an army of advanced laboratory equipment or automated diagnostic workflow setups for the various steps, and thus prevalent in centralized facilities testing samples in large batches. Consequently, the turn-around time for these tests has proven to be 24-48 hours globally.
Here, we propose to develop a new Covid-19 test, 1-SWITCH, consisting of a single step switchable fluorescent probe assay. The 1-SWITCH technology will use low cost reagents, and therefore will be cheaper than current available Covid-19 tests. The sensitivity of the test will be superior to the one of antigen tests and comparable to the one of PCR tests. This 1-step, 1-tube method will detect the virus directly within minutes and eliminates the assay complexity, thus facilitating smoother workflows in laboratory diagnostics as well as point of care screening. This will consist of a major breakthrough in clinical diagnostics. Plans for bringing our research finding to a commercial innovation are presented.

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Topic(s)

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ERC-POC - Proof of Concept Grant

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Call for proposal

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(opens in new window) ERC-2020-PoC

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Host institution

LUNDS UNIVERSITET
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 150 000,00
Address
Paradisgatan 5c
22100 Lund
Sweden

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Region
Södra Sverige Sydsverige Skåne län
Activity type
Higher or Secondary Education Establishments
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Total cost

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Beneficiaries (1)

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