Skip to main content

NEW METHODS FOR THE SELECTION OF RAW MATERIALS AND THE CONTROL AND MONITORING OF MICROBIOLOGICAL PARAMETERS WITHIN THE BREWING INDUSTRY

Objective

Motivated by the industrialization of the brewing process and the internationalization of its markets, the brewing industry has been seeking to improve process efficiency and product quality.

Beer manufacturing is a complex procedure in which several highly variable raw materials are fermented by microorganisms, which are themselves subject to high variability from batch to batch. A deviation from the normal process has serious consequences on product quality and, in some instances, may result in the loss of a batch. The main shortcoming of existing analytic methods is that due to their lack of speed, sensitivity and specificity, they fail to provide the brewer with a decision making tool enabling corrective action at the appropriate processing stage. The objective of this project is to develop a complete range of new process control and quality assurance methods based on advanced biotechnology, immunochemistry and novel analytical instrumentation.
The work, based on advanced biotechnology, immunochemistry, and novel analytical instrumentation, addresses the selection and sorting of raw materials for the brewing industry, and the monitoring and control of key aspects of the brewing process itself. Colorimetric assays for malt enzymes were carried out, and the methods assessed on malt extracts and for the monitoring of industrial mashing. A rapid method for electrophoretic identification of barley varieties was also developed. The microflora from barley/malt samples was analysed, and the main field/storage fungi determined. The ChemFlow system was evaluated for determination of brewing yeast concentration, viability and activity. These experiments resulted in a protocol for brewing yeast, which was applied afterwards to the monitoring of laboratory and industrial fermentations.

Chromosome fingerprinting, deoxyribonucleic acid (DNA) and protein staining of yeasts were also investigated, as well as detection of wild yeasts after selective growth. Concerning beer spoiling bacteria, a balanced cocktail of 10 monoclonal antibodies against lactobacilli was developed, to obtain the broadest possible recognition. Numerous strains were tested by slide immunofluorescence, many of them being clearly recognized.

The objective of this project has been to develop a complete range of new process control and quality assurance methods based on advanced biotechnology, immunochemistry and novel analytical instrumentation. The project addresses therefore both the selection and sorting of raw material for the brewing industry and the monitoring and control of key aspects of the brewing process itself.
We have organized and supervized barley microflora identification, as well as assessed yeast chromosome fingerprinting for identification of industrial strains. A protocol has been developed for the monitoring of laboratory and industrial strains. Investigations were also performed on yeast deoxyribonucleic acid (DNA) and protein staining.

The objective of this project has been to develop a complete range of new process control and quality assurance methods based on advanced biotechnology, immunochemistry and novel analytical instrumentation. The project addresses therefore both the selection and sorting of raw material for the brewing industry and the monitoring and control of key aspects of the brewing process itself.
Tepral supplied the source material for production of monoclonal antibodies for barley differentiation and validated the assays. Along with preliminary purifications of thionins, Tepral performed the evaluation of enzymatic assays on malt extracts and their application to the follow up of industrial mashing. Tepral also evaluated the ChemFlow system for monitoring of industrial fermentations.

The objective of this project has been to develop a complete range of new process control and quality assurance methods based on advanced biotechnology, immunochemistry, and novel analytical instrumentation. The project addresses therefore both the selection and sorting of raw material for the brewing industry and the monitoring and control of key aspects of the brewing process itself.
Chemunex develops monoclonal antibodies and assays for barley and microbiological control. Colorimetric enzymatic assays for barley beta-amylase and total alpha-amylase have been implemented. Antibodies were used to determine alpha-amylase I activity in malt. Immunization strategies were performed to elicit sufficient immune response in mice against barley thionins and monoclonal antibodies have been produced. Yeast chromosome fingerprinting was implemented and pitching yeast control set up. Monoclonal antibodies against Lactobacilli and Pediococci were produced and these antibodies were tested by slide immunofluorescence against a collection of bacteria. A cocktail of monoclonal antibodies was subsequently elaborated in order to obtain a broad recognition of Lactobacilli.
The project addresses the selection and sorting of raw materials for the brewing industry and the monitoring and control of key aspects of the brewing process itself. The work will be performed in the following specific areas: barley and malt selection and control; yeast purity and activity control; fermentation control; plant hygiene control; and finished and intermediate product control.

Barley and malt selection and control. Development of enzymatic assays and immunoassays (after production of monoclonal antibodies), for the selection of barley varieties as a function of their proteinic and enzymatic profiles.

Yeast purity and activity control. Determination of yeast purity by detection of bacterial and wild yeast contamination.

Fermentation control. In process control of yeast multiplication and online monitoring of individual cell activity and enumeration of yeast levels in suspension after fermentation and prior to filtration.

Plant hygiene control. In between batches, control of cleaning and disinfection procedures.

Finished and intermediate product control. Development of an immunoassay for specific beer spoiler microorganisms, based on a monoclonal antibody cocktail in an immunofluorometric assay.

Coordinator

Chemunex SA
Address
41 Rue Du 11 Novembre 1918
94700 Maisons-alfort
France

Participants (5)

Alfred Jørgensen Laboratory Ltd
Denmark
Address
30,Frydendalsvej
1809 Frederiksberg C
Bavaria BV
Netherlands
Address
35,Burg. V.d. Henvelstraat
5737 Lieshout
Brouwerijen Alken-Maes NV
Belgium
Address
10,Waarloosveld
2550 Kontich
Central de Cervejas EP
Portugal
Address
115,Av. Almirante Reis
1197 Lisboa
TEPRAL SA
France
Address
Route D'oberhausbergen 20
67037 Strasbourg