Objective
2 outstanding and unresolved problems exist in in vitro plant micropropagation techniques. The lack of knowledge about the composition of the inner gaseous environment of containers and its influence on the physiological behaviour of vitroplants, and the lack of an automated process to reduce the high level of the cost of in vitro techniques.
The following subjects have been addressed:
analysis of the gas phase in containers implanted with vitroplants;
analysis of selective permeability of membranes and adjustment to tissue culture;
development of a disposable plastic container with selected membranes;
biological assays with vitroplants until the acclimatisation phase and scale-up.
Two outstanding and unresolved problems exist with in vitro plant micropropagation techniques. These are a lack of knowledge about the composition of the inner gaseous environment of containers and its influence on the physiological behaviour of vitroplants, and the lack of an automated process to reduce the high level of the cost of in vitro techniques.
The project will respond to these problems using the following approaches.
A detailed analysis of components of the gas phase inside containers.
The control of the gas phase by using membranes with a selective permeability to gases. Tests of selectivity of membranes, feasibility of technical process for using membranes as lids of containers and for developing a disposable cheap container, will be performed.
Biological assays using the normal containers will be performed with choice of different membranes and permeabilities in different culture conditions (gelled and artificial substrates, solid and liquid nutrient media). A large spectrum of plant species (Gymnosperms, Angiosperms, Monocots, Dicots, herbaceous, and woody plants) and plant tissue cultures forms will be tested.
If the results are consistent with the objectives, many applications would be available in the wide field of in vitro techniques of plant micropropagation.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
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Coordinator
21110 Genlis
France
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