Objective
The aims of the project are:
to establish a map of the parasite presence in both natural and culture areas of Ostrea edulis
to identify a possible carrier host
to test the influence of the environmental factors and the culture conditions
to test the influence of the stress on the development of the disease
to select the resistant strains to the disease
to study the mechanisms host-parasite of identification, elimination and escape
The development of this project aims to propose practical solutions in order to preserve and stimulate the culture of the plat oyster in the EEC countries.
Bonamia ostreae, an intrahaemocytic protozoan parasite infecting the oyster Ostrea edulis, is responsible for an endemic disease affecting all intensive breeding areas of European countries. Epidemiological studies showed variables prevalences, 43% in Galicia (Spain), from 6% to 32% in France and 45% in the most important area of Ireland.
The impact of oyster transfers, handlings and high densities have been implicated in the disease development but environmental stressors could be more involved than zootechnical methods. The effects of water temperature, salinity and tidal exposure were particularly examined to assess stress induced changes and determine physiological, biochemical and immunological state indices of oyster populations, which will contribute to progress in the establishment of prophylactic measures.
With Bonamia purification protocol, experimental infections have permitted to study bonamiasis development kinetics, confirming a latent period with unknown location of the parasite within the animal tissues. Concerning haemocyte parasite interaction studies, using in vitro haemocyte infections and a chemiluminescence technique, it has been shown that Bonamia counteracts the oxidative defence system in both O edulis and C gigas.
The question of specificity barrier and elimination of B ostreae in Japanese oyster remains unresolved.
Finally, experimental infections allowed a selection program to be undertaken for Bonamia resistant oysters. This could be the most effective strategy to ensure uninterrupted production of flat oysters.
The work will be carried out following this programme:
Descriptive epidemiology
Analytic epidemiology
Study of the stress at cellular and molecular level
Test of the strains: search of a resistant strain
Study of the defence mechanisms.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques.
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17390 La Tremblade
France