Objetivo The general aim of this project is to develop new approaches to genetic management and selective breeding in fish by means of recent advances in molecular genetics and chromosome manipulations. Rainbow trout and Atlantic salmon will be chosen as species models. The first step of this project will consist in cloning a large number of DNA probes which will allow to describe the genetic variability at the DNA level. These methods will provide, together with protein polymorphism studies, basic informations which could be immediately applied to: description of gene diversity of domesticated and natural populations and, if necessary, introduction of new genetic material in fish-farm stocks from the wild, evaluation of impact of fish-farm escapes on wild populations, conservation of genetic resources of species. The mid-step goal will be the construction of a preliminary linkage map of the polymorphic enzyme loci and DNA sites detected with the salmonid sequences cloned in step 1 and the long-term goal will be the use of this linkage map for the detection of major genes coding for quantitative traits in rainbow trout and Atlantic salmon. The biological material used for these two goals will be full-sib families and homozygous rainbow trout lines which will be obtained by chromosome manipulations. The project can be divided into three major interconnected phases: DNA probe cloning, description of RFLPs (restriction fragment length polymorphism) and enzyme variation, construction of a preliminary linkage map and analysis of linkage between RFLPs and QTLs (quantitative trait loci). Ámbito científico natural sciencesbiological sciencesmolecular biologymolecular geneticsnatural sciencesbiological sciencesgeneticsDNAnatural sciencesbiological sciencesgeneticschromosomesnatural sciencesbiological sciencesgeneticsgenomesnatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsenzymes Programa(s) FP2-FAR - Community research and coordination programmes (EEC) in the fisheries sector, 1988-1992 Tema(s) Data not available Convocatoria de propuestas Data not available Régimen de financiación Data not available Coordinador Institut National de la Recherche Agronomique (INRA) Aportación de la UE Sin datos Dirección Domaine de Vilvert 78352 Jouy-en-Josas Francia Ver en el mapa Coste total Sin datos Participantes (4) Ordenar alfabéticamente Ordenar por aportación de la UE Ampliar todo Contraer todo Institut National de la Recherche Agronomique (INRA) Francia Aportación de la UE Sin datos Dirección Domaine de Vilvert 78352 Jouy-en-Josas Ver en el mapa Coste total Sin datos UNIV OVIEDO-FACULTAD DE MADECINA-AREA DE GENETICA España Aportación de la UE Sin datos Dirección Oviedo Ver en el mapa Coste total Sin datos UNIVERSITY COLLEGE GALWAY-DEPARTMENT OF MICROBIOLOGY Irlanda Aportación de la UE Sin datos Dirección Galway Ver en el mapa Coste total Sin datos Unilever UK Central Resources Ltd Reino Unido Aportación de la UE Sin datos Dirección 17 Dove Road MK44 7AA Sharnbrook Ver en el mapa Coste total Sin datos
