Objective
The overall aim of the project is to improve the production of larvae of marine fish, specifically the sea bass (Dicentrarchus labrax) and the sea bream (Sparus auratus), through optimizing their dietary levels of essential (n-3) polyunsaturated fatty acids (PUFA). It is proposed to achieve this aim by enhancing levels of (n-3) PUFA in the larvae's live feed organism (ie nauplii of the brine shrimp, Artemia salina) by prior administration to Artemia nauplii of: emulsions of triglyceride oils enriched in 22:6 (n-3); liposomal preparations of marine phospholipids enriched in 22:6 (n-3); and silicic acid particles of defined sizes containing absorbed lipids enriched in 22:6 (n-3).
The efficacy of the various supplemented Artemia feeds for larval fish will be tested not only by conventional measurements of length, wet weight and dry weight of the fed fish, but also by direct analyses of the (n-3) PUFA contents of both the whole fish and selected critical tissues including the brains and eyes. These analyses will involve microdissection and will not be easy to perform routinely. However, recent researches have shown an extremely high concentration of (n-3) PUFA, especially 22:6 (n-3), in the brains and eyes of larval fish (eg some 50% of the total fatty acids of phospholipids in fish eyes is 22:6 (n-3)). Therefore, these tissues, whose normal development is critical for the successful performance of predatory fish larvae, will provide very sensitive indicators of the fish's (n-3) PUFA status. Finally, a simple stress test will be applied to the developing larvae, namely recording survival following removal of the fish from seawater for periods up to 60 seconds and subsequent return to eawater.
Research on dietary supplementation and protocols, together with lipid analyses of the Artemia and fish larvae, will be conducted at Stirling. Feeding trials will be conducted at Castellon.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
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Programme(s)
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Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
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Coordinator
FK9 4LA Stirling
United Kingdom
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