Objective
THE SCIENTIFIC COMMUNITY IS BEING PROVIDED WITH AUTHENTICATED PLASMID BEARING STRAINS, MUTANTS AND HOST STRAINS OF BIOTECHNOLOGICAL IMPORTANCE.
THIS SERVICE WILL BE MADE WIDELY KNOWN THROUGH THE CATALOGUE OF THE COLLECTION, EUROPEAN CULTURE COLLECTIONS' ORGANIZATION (ECCO), WORLD FEDERATION FOR CULTURE COLLECTIONS (WFCC), MICROBIAL INFORMATION NETWORK EUROPE (MINE), AND OTHER AVENUES.
European culture collections have lacked centres with the specialized skills necessary to handle plasmid bearing strains, and thus there were no provisions for their collection, preservation and distribution. The aim was to establish centres with quality control procedures suitable for collections of plasmid bearing strains of biotechnological and medical importance, including patent strains.
Methods and quality control procedures were developed which allow the freeze drying of plasmid bearing strains without loss or alteration of their characteristics. The means of quality control established include: before and after freeze drying checks on stability of host cells, plasmid presence and absence, molecular weight of plasmids, confirmation of phenotypic markers and, where appropriate, restriction digests of plasmid deoxyribonucleic acid (DNA).
Seed stocks for rebatching are kept in liquid nitrogen, and plasmid bearing strains are distributed in freeze dried ampoules.
The databases established at each collection are an information resource and greatly facilitate the production of catalogues and searches.
TO COLLECT AND TO MAKE AVAILABLE TO THE PUBLIC:
PLASMID CARRYING STRAINS OF PROKARYOTES AND EUKARYOTIC MICROORGANISMS OF GENERAL SCIENTIFIC AND BIOTECHNOLOGICAL RELEVANCE.
CLONING VECTORS (PLASMIDS,COSMIDS,PHAGES).
PROKARYOTIC AND EUKARYOTIC PLASMID-FREE HOST STRAINS.
GENETICALLY MANIPULATED PLASMIDS INCLUDING DEPOSITS FOR PATENT PURPOSES.
THE DISTRIBUTION OF THE LATTER MAY BE RESTRICTED ACCORDING TO PATENT LAW.
CATABOLIC PLASMIDS.
PLASMID STANDARDS FOR MOLECULAR WEIGHT DETERMINATION.
MICROORGANISMS KNOWN TO PRODUCE SPECIFIC RESTRICTION ENDONUCLEASES.
GENE BANK (IN SPECIAL CASES, IF NEEDED).
TO STUDY:
LONG-TERM MAINTENANCE METHODS (FREEZE-DRYING,FREEZING IN LIQUID NITROGEN) FOR PLASMID BEARING BACTERIAL STRAINS AND ISOLATED PLASMID DNA TO ENSURE THAT PRESERVATION DOES NOT LEAD TO A LOSS OF PLASMIDS.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences computer and information sciences databases
- natural sciences biological sciences microbiology virology
- natural sciences biological sciences genetics DNA
- social sciences law
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
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Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
38124 BRAUNSCHWEIG
Germany
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.