Objective
DEVELOPMENT OF NEW ENZYME REACTORS FOR CONTINUOUS PRODUCTIONS OF WATER SOLUBLE AND WATER INSOLUBLE PRODUCTS REQUIRING REGENERATION OF REDOX COFACTORS.
THE FOLLOWING CHEMICALS WILL BE PRODUCES :
1. L-ALANTINE, L-METHIONINE,
2. LONG CHAIN ALDEHYDES,
3. LACTIC AND ACID FROM MALIC ACID,
4. HIGH-ADDED-VALUE LIPIDS.
The development of many industrial enzymatic processes is dependent upon the discovery of thermostable enzymes (ie those extracted from extremophiles) or methodologies for activity stabilization (ie immobilization) in the presence of organic solvents, with coenzyme regeneration.
Research was carried out in order to contribute to solving the general problem of enzyme stability and coenzyme regeneration in aqueous or mixed solvent and water solutions, in enzyme reactors and in liquid membranes.
The potentiality of some extremophilic organisms' cells and enzymes which are thermostable and resistant to common protein denaturing agents and to organic solvents was studied. These enzymes were purified and produced by genetic engineering.
A new alcohol dehydrogenase (ADH), a beta-galactosidase and a malic enzyme were purified from the extremophile Sulfolobus solfataricus. These thermostable and solvent resistant enzymes were used for the synthesis of long chain aldehydes, chiral compounds, L-amino acids and for malic acid conversion and lactose hydrolysis.
2 gene banks were constructed and the beta-galactosidase gene was isolated from the extremophile, cloned, sequenced and expressed in Escherichia coli. New types of solid gas bioreactors were developed and have been used with coenzyme regeneration for the synthesis of aldehydes or transesterification reactions catalyzed by lipases, whose activities and regioselectivities were investigated in different systems (eg microemulsions, etc) with and without immobilization. L-amino acid and aldehyde productions were investigated in liquid membranes with coenzyme regeneration.
Extremophile enzymes appear to be different with little or no homology with the corresponding ones from mesophiles; they are thermostable and solvent resistant proteins whose structures can be used as models for producing proteins by protein engineering.
USE OF A NEW THERMOSTABLE, SOLVENT RESISTANT ALCOHOL DEHYDROGENASE FOR THE CONTINUOUS PRODUCTION OF FOOD ADDITIVES WITH PERMANENT COFACTOR REGENERATION.
HORSE LIVER ALCOHOL DEHYDROGENAGE AND THERMOPHILIC ALCOHOL DEHYDROGENASE ACTIVITIES BIOREACTORS WITH NAD AS COFACTOR FOR THE PRODUCTION OF LONG CHAIN ALDEHYDES. THE ENZYMES AND COFACTOR WILL BE IMMOBILIZED INTO A PROTEIC FOAM WHILE INSOLUBLE SUBSTRATES AND IMMISCIBLE SOLVENT WILL CONSTITUTE THE ORGANIC PHASE. THE BEST SYSTEM WILL BE OPTIMIZED IN AN EXPERIMENTAL COLUMN PACKED REACTOR AND THEN SCALED UP.
THE LIQUID MEMBRANE REACTOR WILL ALSO BE TESTED. ALSO DIFFERENT LIPASES ACTIVITIES WILL BE TESTED IN MICROEMULSIONS TO INCREASE THE INTERFACE BETWEEN THE AQUEOUS AND ORGANIC MEDIA. TRANSESTERIFICATION AND SYNTHESIS REACTIONS WILL BE SEARCHED IN ORDER TO OBTAIN HIGH ADDED VALUE LIPIDS FROM OLIVE OIL.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques.
- engineering and technologyenvironmental biotechnologybioremediationbioreactors
- natural scienceschemical sciencesorganic chemistryaldehydes
- natural sciencesbiological sciencesbiochemistrybiomoleculeslipids
- natural scienceschemical sciencesorganic chemistryalcohols
- natural sciencesbiological sciencesbiochemistrybiomoleculesproteinsenzymes
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CSC - Cost-sharing contractsCoordinator
60206 Compiègne
France