Objective THE CELL CULTURE AND GENETIC MANIPULATION OF 4 ECONOMICALLY IMPORTANT CROP PLANTS WILL BE EXAMINED, AND ALL DEVELOPMENTS ACHIEVED WITH A MODEL SPECIES (NICOTIANA PLUMBAGINIFOLIA) WILL BE APPLIED TO THE CROP SPECIES BY THE FOLLOWING LABORATORIES : - POTATO AND RELATED SPECIES : HARPENDEN, GRONINGEN, WAGENINGEN. - TOMATO : PAVIA. - SUGAR BEET : BRUSSELS. - RAPE AND RELATED SPECIES : VERSAILLES. THE ULTIMATE OBJECTIVE BEING THE INTRODUCTION INTO CLASSICAL PLANT BREEDING PROGRAMMES OF UNCONVENTIONAL CELLULAR AND MOLECULAR APPROACHES OF GENETIC TRANSFORMATION. GENETIC MANIPULATION OF PLANT CELLS EMPLOYS AN ARRAY OF METHODS AND TECHNIQUES FOR THE TRANDFER OF GENETIC ELEMENTS AND COMPLEXES VARYING FROM WELL DEFINED DNA FRAGMENTS INCORPORATED INTO PLANT DNA VECTORS TO COMPLETE NUCLEI BY MEANS OF CELL FUSION. IN BETWEEN THE TWO EXTREMES, OPTIMAL TRANSFER OF E.G. NAKED DNA AND CELL ORGANELLES REQUIRES THE FURTHER DEVELOPMENT OF CELLULAR ENGINEERING FOLLOWED BY INVESTIGATIONS INTO INTEGRATION AND EXPRESSION OF THE TRANSFERRED MATERIAL IN THE RECIPIENT CELL. THE DEVELOPMENT OF THIS APPROACH IS THE CENTRAL PART OF THIS PROPOSAL. IN AN INTEGRATED APPROACH, THE FOLLOWING METHODS FOR GENETIC MANIPULATION OF CROP PLANTS WILL BE EXAMINED : - ISOLATION AND CHARACTERIZATION OF SELECTABLE MUTANTS (BRUSSELS, PAVIA, VERSAILLES). - ANALYSIS OF THE CELL CYCLE AND SYNCHRONISATION (WAGENINGEN, PAVIA, VERSAILLES). - CELL-MEDIATED TRANSFER (HARPENDEN, GRONINGEN, BRUSSELS, PAVIA). - CHROMOSOME-MEDIATED TRANSFER (WAGENINGEN). - DNA-MEDIATED TRANSFER (HARPENDEN, GRONINGEN, BRUSSELS, PAVIA). - LIPOSOME-MEDIATED TRANSFER (WAGENINGEN, VERSAILLES). - REGENERATION TECHNIQUES (HARPENDEN, PAVIA). - CYTOLOGICAL AND MOLECULAR ANALYSIS OF PUTATIVE TRANSFORMANTS (ALL LABORATORIES). Fields of science natural sciencesbiological sciencesgeneticsDNAmedical and health sciencesmedical biotechnologygenetic engineeringagricultural sciencesagriculture, forestry, and fisheriesagricultureagronomyplant breeding Programme(s) FP1-BAP - Multiannual research action programme (EEC) in the field of biotechnology (BAP), 1985-1989 Topic(s) Data not available Call for proposal Data not available Funding Scheme CSC - Cost-sharing contracts Coordinator VRIJE UNIVERSITEIT BRUSSEL Address Labo. voor plantengenetica paardenstraat 65 1640 Rhode-saint-genese Belgium See on map EU contribution € 0,00 Participants (5) Sort alphabetically Sort by EU Contribution Expand all Collapse all INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE France EU contribution € 0,00 Address Route de saint-cyr rd10 78026 Versailles See on map RESEARCH INSTITUTE ITAL Netherlands EU contribution € 0,00 Address Wageningen See on map ROTHAMSTED RESEARCH LIMITED United Kingdom EU contribution € 0,00 Address West common AL5 2JQ Harpenden, herts See on map UNIVERSITA DEGLI STUDI DI PAVIA Italy EU contribution € 0,00 Address 271 Pavia See on map UNIVERSITY OF GRONINGEN Netherlands EU contribution € 0,00 Address Haren See on map
