Objective
THE POSSIBILITY TO ALTER THE GENETICS OF DOMESTIC ANIMALS AND FISH IN A PREDICTABLE MANNER IS CLEARLY VERY ATTRACTIVE :
- SCIENTIFICALLY, THIS RESEARCH SHOULD CONTRIBUTE TO A BETTER UNDERSTANDING OF A FOREIGN GENE IN THE GERM LINE OF ANIMALS.
- TECHNICALLY, IT SHOULD IMPROVE THE PRACTICAL METHODS OF TRANSER.
- ECONOMICALLY, IT SHOULD PROVIDE A SHORT CUT FOR THE IMPROVEMENT OF THE GENETICS OF ANIMALS, FOR INSTANCE IN OBTAINING ANIMALS GROWING AT A FASTER RATE.
Genetic characteristics of domestic animals can be rapidly modified through the transfer of deoxyribonucleic acid (DNA) to fertilized ova and the development of transgenics. Biological fluids (milk, blood, egg white, etc) of transgenic animals can be an abundant source of well matured proteins of clinical and veterinary interest. These goals can be reached if specific genes, promoters and vectors are prepared, and if methods to obtain transgenic animals in various species are defined.
Recombinant genes containing different promoters and growth hormone genes were prepared and tested in cultured mammalian and fish cells and in whole transgenic salmonids. Most of the regulatory elements from mammalian genes when tested in fish cells or in fish worked only on low levels. Fish genepromoters seem to be a better choice for the future. Several fish genes were cloned and characterized. Rabbit milk and bovine complementary deoxyribonucleic acid (cDNA) and genes were isolated and sequenced. Recombinant genes containing milk gene or bovine liver gene promoters and reporter genes were expressed in mammary cells in vitro and in transgenic mice. Retroviral vectors from birds were prepared. These vectors harbouring foreign genes transfer efficiently to chickens. Conditions were established for the efficient recovery of fertilized bovine ova, their microinjection, in vitro culture and transfer to recipients, with a high rate of survival. Finally, protocols were established for efficient generation of transgenic fish.
TWO STRATEGIES WILL BE USED TO INTRODUCE A NUMBER OF GENES INTO THE GERM LINE OF THE ANIMALS :
- MICROINJECTION OF DNA,
- DELIVERY OF THE DNA BY RETROVIRUSES.
THREE GENES ARE ALREADY AVAILABLE : MOUSE METALLOTHINEIN, AND THE MMTV AND SV40 PROMOTERS. IT IS PROPOSED TO CLONE PROMOTERS FOR : HIGH LEVEL OF CALF PROTEIN SEQUENCE, HIGH LEVEL OF A SALMON PROTEIN SEQUENCE, THE RABBIT CASEIN PROMOTER AND THE SALMON AND BOVINE GROWTH HORMONE PROMOTERS.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- medical and health sciences medical biotechnology genetic engineering gene therapy
- natural sciences biological sciences genetics DNA
- agricultural sciences animal and dairy science domestic animals
- natural sciences biological sciences biochemistry biomolecules proteins
You need to log in or register to use this function
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Data not available
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Data not available
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
69365 LYON
France
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.