Objective
S.AUREUS IS ONE OF THE MAJOR CAUSES OF BOVINE MASTITIS. THE LONG TERM AIM OF THIS WORK IS TO IDENTIFY THE VIRULENCE DETERMINANTS IN S.AUREUS FROM WHICH A VACCINE AGAINST MASTITIS CAN BE MADE.
Antibiotic therapy is largely ineffective and no adequate vaccine is available. S aureus expresses a variety of factors (eg toxins, enzymes, surface components) that might be important in infection and could be components in a future vaccine.
Techniques for contructing site specific mutations in S aureus were devised. Mutants defective in alpha-toxin, beta-toxin and protein A were constructed and were shown to be less virulent than the wild type in the mouse mastitis infection model, providing clear evidence that these factors are important in pathogenesis. Expression of alpha-toxin caused mortalities in infected animals and was the major cause of damage to cells (including phagocytes). Expression of beta-toxin promoted growth in vivo, whereas protein A helped bacteria avoid being phagocytosed. In contrast, coagulase deficient mutants were still virulent.
THE SPECIFIC AIM OF THIS PROJECT IS TO CLONE AND TO INACTIVATE BY SITE-DIRECTED MUTAGENESIS THE GENES SPECIFYING PROTEIN A, COAGULASE AND B-HAEMOLYSIN PRODUCTION BY S. AUREUS (THIS PART TO BE DONE BY T.J. FOSTER, DUBLIN). THE VIRULENCE OF THESE MUTANTS WILL BE ASSESSED IN THE MAMMARY GLAND OF THE MOUSE BY DR. BRAMLEY'S TEAM.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- social sciences sociology demography mortality
- natural sciences biological sciences microbiology bacteriology
- medical and health sciences basic medicine pharmacology and pharmacy pharmaceutical drugs vaccines
- natural sciences biological sciences genetics mutation
- natural sciences biological sciences biochemistry biomolecules proteins enzymes
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
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Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
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Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
RG16 0NN NEWBURY
United Kingdom
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.