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ADVANCED MONITORING AND COMPUTER CONTROL OF BIOTECHNOLOGICAL PROCESSES

Objective

WORK STATION FOR BIOENGINEERS FOR THE EXPERIMENTATION, ANALYSIS AND CONTROL OF BIOTECHNOLOGICAL PROCESSES.

COMBINED WITH A RELIABLE PILOT PLANT IT WILL PERMIT THE TESTING AND THE EVALUATION OF MONITORING AND CONTROL STRATEGIES OF BIOREACTORS, LEADING TO A MORE EFFICIENT BIOREACTOR DESIGN.
The application of modern control science to the optimization of biotechnological processes comes up to against 2 major problems: the difficulty of representing the dynamics of living microorganisms by validated mathematical models; the lack of cheap and reliable instrumentation for online measurement of the biological variables required to implement high performance automatic control strategies.

Research was carried out in order to develop an integrated set of algorithms and software tools for automatic monitoring and computer control of biotechnological processes with a view to yield optimization. Based on mathematical models of the plant dynamics the tools use specific instrumentation.
The algorithms are based on the most recent advances of nonlinear systems theory, in a way adapted to the specific biological characteristics of bioprocesses.

New devices for online monitoring of bioprocesses have been developed involving an automatic sensor for bicarbonate measurement, an in situ sterilizable interface for the online connection of bioreactors to sophisticated analysers and a flow injection analysis system for online glucose measurement.
New algorithms for advanced monitoring and control of bioprocesses have been developed for software sensors for online estimation of biological parameters that are not accessible through direct measurements and for robust nonlinear (adaptive) controllers for the stabilization and the optimization of biological variables (substrates and metabolites) in bioreactors. A dedicated workstation named CAMBIO has been designed for interactive computer aided modelling, simulation and experimental design of processes.
Validation on pilot and industrial plants have covered a wide range of potential applications involving pharmaceutical and biomedical products, food industries and biological waste treatment.
DEVELOPMENT AND PROVISION OF "BIO-INFORMATICS" TOOLS (ALGORITHMS, SOFTWARE AND HARDWARE) FOR AUTOMATIC MONITORING AND COMPUTER CONTROL OF BIOREACTORS AND BIOTECHNOLOGICAL PROCESSES WITH A VIEW TO YIELD OPTIMIZATION AND PRODUCTS QUALITY IMPROVEMENT. THESE TOOLS WILL BE RATIONALIZED SO AS TO ACCOMODATE A BROAD CLASS OF BIOLOGICAL MODELS AND INDUSTRIAL PROCESSES. THEY WILL BE VALIDATED BY SIMULATION AND ON PILOT PLANTS.

WORK AT IRSA WILL CONCENTRATE ON THE DEVELOPMENT OF MONITORING STRATEGIES BASED ON PHYSICO-CHEMICAL MEASUREMENTS. IN PARTICULAR, BICARBONATE AND CARBON DIOXYDE SENSORS WILL BE DEVELOPED AND TESTED AND WILL BE INTEGRATED IN AN OPTIMIZED PROCESS CONTROL SYSTEM.

Funding Scheme

CSC - Cost-sharing contracts

Coordinator

Istituto di Ricerca sulle Acque
Address
Via De Blasio 5
70123 Bari
Italy

Participants (3)

Institut National Polytechnique de Grenoble
France
Address

Grenoble
Institut National de la Recherche Agronomique (INRA)
France
UCL
Belgium
Address

Louvain-la-neuve