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PRODUCTION OF GLYCOPOLYPEPTIDE ANTIGENS OF PORCINE TRANSMISSIBLE GASTROENTERITIS VIRUS

Objective

OBTENTION OF A VACCINE AGAINST TRANSMISSIBLE GASTROENTERITIS OF PIG.
Studies were aimed at identifying, characterizing and defining the roles of the gene products of poraine transmissable gastroenteritis virus (TGEV) in pathogenesis and immunity by means of recombinant deoxyribonucleic acid (DNA) technology. Two of the viral strucutral proteins were glycosylated and one of them, the peplomer, contained regions to which antiboides could attach, thereby preventing viral growth. In order to express the peplomer protein and identify regions involved in immunity, the isolation, sequencing and expression of TGEV genes in recombinant viruses and in yeast was carried out. Seven TGEV genes were identified and sequenced. Three of the gene products, nucleoprotein, integral membrane protein and peplomer, were involved in the structure of the virus particle and have been expressed in bacteria, yeast and vaccinia virus. Specific antibodies were raised against the structural proteins and these led to the identification of a specific antibody binding site on the nucleoprotein and several specific sites on the peplomer. The peplomer protein expressed by vaccinia virus still reacted with all the specific antibodies and the relative positions of 4 antigenic sites were identified on the peplomer protein. Antisera to the peplomer expressed by vaccinia appeared to prevent viral growth and disease in piglets.
THE OBJECTIVE OF THE RESEARCH IS TO CLONE THE MAIN STRUCTURAL PROTEINS OF THE TRANSMISSIBLE GASTROENTERITIS VIRUS, TO OBTAIN THE EXPRESSION IN YEAST OR IN ESCHERICHIA COLI. PURIFICATION OF THE PROTEINS OBTAINED AND TESTING OF THEIR IMMUNOLOGICAL PROPERTIES.

Funding Scheme

CSC - Cost-sharing contracts

Coordinator

UNIVERSITAD DE OVIEDO
Address
Plaza De Riego 4
33071 Oviedo
Spain

Participants (1)

Agriculture and Food Research Council (AFRC)
United Kingdom
Address

Newbury