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Content archived on 2024-04-15

NEPHROTOXICITY MECHANISMS USING KIDNEY PERFUSION AND ANIMAL AND HUMAN RENAL TISSUE AND CELLS

Objective


An investigation has been carried out on nephrotoxicity of drugs using in vitro methods, such as the intact but isolated perfused kidney, freshly isolated fragments representing the glomeruli and proximal tubules, primary cell cultures derived from these cells and cell lines derived from renal cells. The cells in these different systems were studied to assess how the presence of chemicals affected their function and structure. The study confirmed that several chemicals which damage discrete cell types in vivo, also adversely affect the same cell types in vitro. Similarly, if they do not damage a particular cell type in vivo, they are less likely to affect such cells in vitro. This means that the appropriate choice of renal cell types in vitro can indicate the potential nephrotoxicity of new chemcials. The use of appropriate cell types also enabled identification of early events in cellular injury that explain the basis of a chemical targeting for one cell type and not another. This may provide a rational way of limiting renal injury where life saving, but nephrotoxic, drugs have to be used. Progress was also made in using the simple kidney apparatus of the hagfish as a way of understanding the complex processes that occur in the mammalian kidney. Pig kidneys from abattoirs provided a large quantity of tissue for the isolation of cells as an alternative to laboratory animals.

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Funding Scheme

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Coordinator

HANNOVER MEDICAL SCHOOL
EU contribution
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Address
CARL-NEUBERG-STRASSE 1
30625 HANNOVER
Germany

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Total cost

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