Objective
THE POTENTIAL IMPACT CONCERNING DATA CAPTURE AND UPDATING OF DATA BANKS FOR BIOTECHNOLOGICAL/BIOMEDICAL ADVANCEMENT IS EXCEPTIONALLY LARGE. A LASER MICROSCOPE WITH A RESOLUTION BELOW 100 NM AND THREE-DIMENTIONAL DISPLAY COULD HAVE GREAT IMPACT ON BIOTECHNOLOGY AND FIND WIDESPREAD APPLICATION. SPECIFIC ADVANTAGES OVER ELECTRONIC MICROSCOPY INCLUDE : NO NEED FOR VACUUM, THE USE OF LIVE SPECIMENS IN THEIR NATURAL ENVIRONMENTS; AND THE ABILITY TO STUDY SPECIMEN DEVELOPMENT DURING TIME. THERE IS A POTENTIALLY LARGE MARKET IN WHICH EUROPE WOULD TAKE THE LEAD AND THUS EMPLOYMENT TO BE CREATED IN THE EVENT OF SUCCESSFUL DEVELOPMENT.
Starting with the instrument in Amsterdam, the program had the objectives of developing suitable 3-dimensional image processing and routine analyses for extracting the maximum amount of information from the confocal data, and 3D superresolution approaches based on a combination of physical and mathematical techniques available. These efforts were supported on the optical and technical side by the development of special objects, and test specimen and wavefront measurements, while biological test specimens and preparation techniques were supplied from Utrecht.
Using a prototype laser confocal microscope existing in Amsterdam, research was carried out in order to expand the scope and range of application of this type of microscopy. The addition of novel imaging modes, superresolution techniques and dedicated 3-dimensional image processing would make this technique a powerful analytical tool for biology.
A double detector system was added to the Amsterdam instrument for multiple label detection in biological systems. Use of variable microdiaphragms in illumination and detection provided optical probe control. Data inversion methods were developed which use a Fourier transform and Tikhonov regularization. These methods were applied to images of biological samples and increased confocal axial resolution was achieved. A novel method was conceived and tested experimentally at low NA for achieving confocal superresolution by holographic processing of the signals in the detection plane. Special optics were developed. A dedicated 3-dimensional image processing and analysis package was developed for the processing of confocal images.
THE PROJECT IS A MULTI-NATIONAL COLLABORATION BETWEEN A NUMBER OF LEADING RESEARCH AND INDUSTRIAL ESTABLISHMENTS WHOSE LONG-TERM OBJECTIVE IS TO ADVANCE SIGNIFICANTLY THREE-DIMENTIONAL AND SUPER-RESOLUTION SCANNING LASER MICROSCOPY. THE BACKGROUND IS THE EXISTING PROTOTYPE CONSTRUCTION OF A LASER-BASED CONFOCAL SCANNING OPTICAL MICROSCOPE AT THE CONTRACTORS LABORATORY. THE SPECIFIC AIM OF THE PROJECT IS TO INTRODUCE A WIDE RANGE OF NEW TECHNIQUES INTO SCANNING LASER MICROSCOPY, BASED ON THE ABOVE MENTIONED SYSTEM. SIX OTHER GROUPS WILL PARTICIPATE IN THE PROJECT (AS SUB-CONTRACTOR) THUS AIMING AT THE ENHANCEMENT OF THE THREE-DIMENSIONAL AND SUPER-RESOLVING (BELOW 100 NM) CAPABILITIES.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences physical sciences optics microscopy super resolution microscopy
- natural sciences physical sciences optics microscopy confocal microscopy
- social sciences economics and business business and management employment
- natural sciences physical sciences optics laser physics
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
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Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Data not available
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
AMSTERDAM
Netherlands
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.