Objective
Methylamine dehydrogenase from Thiobacillus versutus was obtained as a homogeneous preparation. From the molecular weight and studies on the subunits, it appears that it has an alpha 2 beta 2 structure. Homogeneous preparations of the alpha and beta subunits were obtained and by using specific proteases, peptides prepared by high pressure liquid chromatography. A number of peptides were sequenced, providing information to construct probes to clone the genes, to elucidate the amino acid sequence around the attachment sites of the cofactor to the protein chain, and to support the work on the 3-dimensional structure elucidation. Results prove that the cofactor in the enzyme from Th versutus is also tryptophan tryptophyl quinone (TTQ). Attempts to crystallyze the enzyme were successful which led to the first 3-dimensional structure elucidation of a quinoprotein enzyme. The use of crystals treated with inhibitors has shown that the reactive carbonyl group is situated at the C6 carbon atom.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences chemical sciences organic chemistry amines
- natural sciences biological sciences biochemistry biomolecules proteins enzymes
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
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Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
9700 AB GRONINGEN
Netherlands
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.