Objective
P. aureofaciens SBW25, a plasmid free, nonpathogen, was the dominant species (<30%) of bacteria found in the phylloplane of sugar beet. SBW25 was chromosomally marked with pMOB::TN5XYLE, to confirm the suitability of SWB25, the markers (Kmr, XylE) and to develop the plant model. Recombinants' free of any mobilisable elements or plasmid sequence were made; SBW25 marked by the insertion of a Kmr gene (From Tn903) into a known, nonfunctional chromosomal site to create safe, environmentally acceptable genetically modified organisms (GMOs) as competitively fit as the wild type. Recombinants were indistinguishable from wild type in mono or mixed cultures, with no loss of phenotype recorded over 500 generations in continuous culture of 250 days In planta. SBW25 marked at the Ee site with an integration vector (pBGS)(SBW25EeKmXylE) was equally stable.
A reliable method of transforming B. thuringiensis was developed. The segregational stability of the markers XylE and Gus, positioned on plasmid molecules under the control of Spo2 or PRLB promoters, was assessed in B. thuringiensis, Gus was unstable and therefore unsuitable, XylE and Mer were the preferred non-antibiotic markers.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
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Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
9000 GENT
Belgium
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.