Objective
In general, plasmid vectors are not suitable for environmental studies. They are unstable in the absence of continued selection pressure and they can be horizontally transferred to other bacteria. In contrast, transposon vectors have enhanced stability and reduced horizontal transfer. Therefore Tn5 derivatives were constructed in which the antibiotic resistance genes are replaced by the pupA gene. The delivery vehicle pDK8 is based on a ColE1 replicon and cannot replicate in Pseudomonas. 20% of the transpositions of this transposon occurs without concomitant transposition of the transposase genes, resulting in very stable insertions. This Tn5 pupA transposon has been integrated into the chromosome of the fluorescent Pseudomonas strain Al24. The insertions mutants express the PupA protein and have the ability to metabolise iron(III) complexed to pseudobactin 358. This Tn5 pupA transposon vector therefore can be used to label strains with a selection marker or to introduce foreign genes into the chromosome of a strain. The strains constructed were tested in soil and rhizosphere in greenhouse trials. Data was obtained on colonization, survival, persistence and competition. No differences between wild type strains and genetically modified strains were observed sofar. The rhizosphere was found to be a major determinant in the selective amplification and survival of specific Pseudomonas spp.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences microbiology bacteriology
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences biological sciences genetics chromosomes
- medical and health sciences basic medicine pharmacology and pharmacy drug resistance antibiotic resistance
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
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Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
Netherlands
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.