Objective
The beta-galactosidase (lacZ) gene can be used reliably as a marker to detect and monitor baculovirus recombinants.
The juvenile hormone esterase (JHE) gene can be reliably used to estimate levels of foreign gene expression in insect cell culture and insect larvae. In some viruses it enhances the insecticidal activity of the baculovirus by reducing the weight gain in the feeding larvae. The host range of the recombinants remained unaltered.
A variety of transfer vectors (for polyhedrin and p10 replacement) were constructed to mediate and facilitate the introduction and detection of foreign genes into baculovirus recombinants. These vectors have been freely distributed to other research groups in Europe and the World. Deletion mutants (p10-minus, polyhedral envelope-minus) have been constructed and should result in reduced persistence of recombinants in the field. These mutants enhance the biosafety of genetically engineered baculoviruses, eg, provided with an insecticidal gene, as they reduce the risk for survival of recombinants in the environment.
A cDNA bank has been constructed from RNA isolated from the brain of fifth instar larvae of Spodoptera exigua as a source of insect hormone genes to be introduced into baculoviruses.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences microbiology virology
- natural sciences biological sciences genetics RNA
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
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Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
6701 DB WAGENINGEN
Netherlands
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.