Objective
A family of isogenic L. lactis strains were constructed and used as gene donors in transfer experiments. They carried the same genetic markers (antibiotic resistance genes were used) but on several different supports:
a self-transmissible plasmid;
a broad host range nontransmissible plasmid, having either a low or a high copy number;
bacterial chromosome.
Recipient strains were either a suitably marked L. lactis strains, used for experiments carried out in fermenters or in cheese, or Enterococcus faecalis strains of human origin, used in the mouse digestive tract.
Gene transfer from L. lactis, which is efficient under optimal conditions, was very infrequent in the 3 model systems examined. It was always detected with a self-transmissible plasmid, rarely with a mobilisable plasmid and never with chromosomally carried genes. These observations suggest that the potential risks associated to gene transfer from genetically modified lactic bacteria are probably small.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- medical and health sciences medical biotechnology genetic engineering gene therapy
- natural sciences chemical sciences organic chemistry organic acids
- natural sciences biological sciences microbiology bacteriology
- natural sciences biological sciences genetics chromosomes
- medical and health sciences basic medicine pharmacology and pharmacy drug resistance antibiotic resistance
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Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
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Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
75007 Paris
France
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