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GENETISCHE SCHADEN NACH BESTRAHLUNG MIT NIEDRIGEN DOSEN

Objective



DEVELOPMENT OF THE MICRONUCLEUS TEST AND THE MICROCOLONY ASSAY SYSTEM FOR ANALYSIS OF GENETIC DAMAGE IN CELLS TREATED WITH EITHER IONIZING RADIATIONS OR ENVIRONMENTAL CHEMICALS. DIFFERENTIATION OF THE CONTRIBUTIONS OF 1-TRACK AND 2-TRACK EVENTS IN MORPHOLOGICAL ALTERATIONS OF NUCLEAR CHROMATIN EXPOSED TO CHARGED PARTICLES.ANALYSIS OF RBE COMPONENTS (LOW ENERGETIC ALPHA PARTICLES) ACCORDING TO 1-TRACK-1-BREAK, 1-TRACK-2-BREAK AND 2-TRACK EVENTS IN MAMMALIAN CELL SYSTEMS. INFLUENCE OF REPAIR OF POTENTIALLY LETHAL DAMAGE ON THESE RBE COMPONENTS.

PREVENTION OF REPAIR OF PRECHROMOSOMAL LESIONS BY PREMATURE CHROMOSOME CONDENSATION (PCC-) TECHNIQUE. DEVELOPMENT OF A NEW METHOD TO INDUCE PCC BY ELECTROFUSION. DETAILED ANALYSIS OF THE PREREQUISITES TO GENERATE CELL HYBRIDS FROM IRRADIATED G1-CELL CULTURES AND M-PHASE CULTURES. OPTIMIZATION OF THE TIMING OF ELECTROFUSION ACCORDING TO THE AIM TO PREVENT REPAIR. ANALYSIS OF CHROMOSOME DAMAGE IN PCC INDUCED MITOSIS IN DEPENDENCE ON THE TIME BETWEEN IRRADIATION AND FIXATION (REPAIR KINETICS). APPLICATION OF ELECTROFUSION-PCC TO TREATMENT WITH CERTAIN ENVIRONMENTAL CHEMICALS (NOT YET DETERMINED IN DETAIL) IN ORDER TO INCREASE SENSITIVITY OF DOSE RESPONSE. COMPARISON OF DOSE RESPONSE RESULTING FROM GENETIC DAMAGE WITH DOSE RESPONSE RESULTING FROM MEMBRANE DAMAGE ACCORDING TO ADDITIONAL DATA FROM CELL ROTATION IN AN ALTERNATING FIELD OF HIGH FREQUENCY (ZIMMERMANN METHOD).

INFLUENCE OF GLUTATHIONE (GSH) AND OTHER NON-PROTEIN BOUND SH CELL COMPONENTS ON REPAIR OF PRECHROMOSOMAL LESIONS INDUCED BY EITHER IRRADIATION OF ENVIRONMENTAL CHEMICALS. INVESTIGATION OF GSH DEPLETION ON REPAIR PERFORMANCE AND REVERSION OF THIS EFFECT AFTER INCORPORATION OF NPSH COMPONENTS ADDED TO THE GROWTH MEDIUM .. APPLICATION OF THE ELECTROFUSION TECHNIQUE TO INCREASE THE PLASMA MEMBRANE PERMEABILITY TO CERTAIN THIOLS AND ENZYMES (EVENTUALLY COOPERATION WITH DR P BRYANT, UNIVERSITY OF ST ANDREWS, SCOTLAND) .

Funding Scheme

CSC - Cost-sharing contracts

Coordinator

GSF - FORSCHUNGSZENTRUM FUER UMWELT UND GESUNDHEIT GMBH
Address
Ingolstaedter Landstrasse 1
85764 Neuherberg
Germany