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Content archived on 2024-04-15

ANALYSIS OF CELL CYCLE RADIOSENSITIVITY IN NORMAL AND MUTANT CELLS USING REPLICATION BANDING TECHNIQUES

Objective


A study has been made of chromosome aberration induction and deoxyribonucleic acid (DNA) replication delay at different stages of the DNA synthesis phase of the cell cycle. Replication banding techniques have been applied to in vitro cultures as a means of assigning cells, cytologically, to their position in the cell cycle at the time of radiation or other clastogenic treatment. Chromosomal aberrations can then be scored in marked cells which can later be unscrambled and replaced into cohorts, in correct chronological order, irrespective of perturbation and mitotic delay, to provide much more reliable quantification.
A STUDY OF CHROMOSOME ABERRATION INDUCTION AND DNA REPLICATION DELAY AT DIFFERENT STAGES OF THE DNA SYNTHESIS PHASE OF THE CELL CYCLE.

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Coordinator

Medical Research Council (MRC)
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20 Park Crescent
W1N 4AL London
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