Objective
The future importance of domestic species is expected to arise especially for their increasing commercial use in the fields of biotechnology and human health The large numbers of cheap embryos are integral to the production of pharmaceutical proteins, xenografting and as models for human disease. The technology currently exists and in-vitro procedures have been most heavily useful in commercial companies involved in animal breeding and biotechnology. Whilst some of the European commercial entreprises have survived, others have f largely because of inadequacies in the current in-vitro techniques associated with embryo production.
The objective of this preparatory award is to radically improve the efficiency in-vitro of oocyte growth maturation and early embryonic development by developing a new process which is able to manipulate the rhythm of oscillar parameters (intracellular calcium ions increase, second messengers) by using a intracelluar signal generator (Puls-Ions) with frequency and amplitude modulator (Ozil & Swann, 1995 J. Physiol, 483).
Two set-up which applies our process have been constructed an are in operation. Preliminary results have demonstrated the suitability of our process. It involves replacing the traditional and passive in-vitro culture methods with a dynamic system able to generates a large range of intracellular signals in the oocyte and/or embryo during the various phases of the reproduction process oocyte growth, maturation, fertilization and development which imitate naturally occurring conditions (calcium ions is a critical regulator of many cellular processes and intracelluar calcium changes occur transiently during th cell cycle).
Links have been established among four partners; with a French company (Sanofi), with an Italian private sector laboratory (LTR-CIZ), with an English public sector laboratory (The Brabaham Institute) and a French public laboratory (INRA) to co- ordinate the production of few set up and organise a demonstration to test this process on different animal species and to broaden the scientific scope of its possible applications. However this high-tech process requires substantial investment (>3MFF) and we are therefore requesting financial assistance from the European Union in order to study the feasibility of the production of few set up and to evaluate its suitability and capacity to resolve the problem of industrial and commercial use of the embryo-technology, An RTD project which applies the technology described above has been submitted (15/3/95) Programme 3.2.1 entitled; Enhancing the science of embryo production - molecular foundation and driving conditions for in-vitro oocyte development. Coordinateur R. Moor. Three partners are common to these requests. However, this proposal differs in that It only concerns the setting of a demonstration project for a new technology.
Should the project be selected, the fact that several machines are available, spread across the oocyte experimentation network would enable research on the development of oocytes to be considerably accelerated through diversifying the tests with a robust and reliable technology. This work would enable Europe to improve its position in relation to the North American continent and to promote European livestock breeds.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques.
- natural scienceschemical sciencesinorganic chemistryalkaline earth metals
- natural sciencesbiological sciencesbiochemistrybiomoleculesproteins
- natural sciencesbiological sciencesdevelopmental biology
- agricultural sciencesanimal and dairy sciencedomestic animalsanimal husbandry
- medical and health sciencesclinical medicineembryology
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Call for proposal
Data not availableFunding Scheme
EAD - Exploratory awards (demonstration)Coordinator
33501 Libourne
France