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Completion of the genome of sulfolobus solfataricus

Objective



THE PRIMARY OBJECTIVE OF THIS PROJECT IS TO CONTRIBUTE TO THE COMPLETION OF THE GENOME SEQUENCE OF THE ARCHAEAL HYPERTHERMOPHILE SULFOLOBUS SOLFATARICUS. THIS PROJECT WAS STARTED IN 1993 BY THREE CANADIAN LABORATORIES SPONSORED BY THE CANADIAN NATIONAL RESEARCH COUNCIL. 25% OF THE 3 MBP GENOME HAS BEEN SEQUENCED AND ABOUT ONE THIRD WILL BE COMPLETED WHEN THEIR 3 YEAR GRANT TERMINATES IN SUMMER 1996. THEY HAVE BEEN PROMISED A THREE YEAR RENEWAL, AT THE SAME FINANCIAL RATE AS BEFORE, WHICH MEANS THAT THEY WILL HAVE SEQUENCED TWO-THIRDS OF THE GENOME BY THE END OF 1999.
WE ARE THREE EUROPEAN UNIVERSITY LABORATORIES AND ONE INDUSTRIAL LABORATORY WHO HAVE NEGOTIATED WITH THE CANADIAN GROUPS THAT WE WILL SEQUENCE THE REMAINING ONE THIRD OF THE GENOME. THE CANADIANS HAVE A LINEAR COSMID LIBRARY COVERING ALMOST THE WHOLE GENOME AND THEY WILL PROVIDE US WITH COSMIDS COVERING ABOUT ONE THIRD OF THE GENOME.
THE THREE EUROPEAN UNIVERSITY LABORATORIES WILL PERFORM SUB-CLONING AND SEQUENCING AND NOVO-NORDISK A/S WILL HELP BOTH WITH PROCESSING OF THE SEQUENCING DATA AND WITH DEVELOPING SOFTWARE FOR ACCESSING REGULATORY SIGNALS, MOTIFS AND CODING REGIONS IN THE GENOME SEQUENCES WHICH ARE OF POTENTIAL INDUSTRIAL INTEREST; THESE THEY WILL MAKE AVAILABLE TO THE INDUSTRIAL PLATFORM. THE NEW SEQUENCES WILL BE EXCHANGED WITH THE CANADIANS AND MADE AVAILABLE, AS QUICKLY AS POSSIBLE (WITHIN A FEW MONTHS), TO UNIVERSITY AND INDUSTRIAL LABORATORIES WORKING ON ARCHAEAL HYPERTHERMOPHILES AND THERMOSTABLE GENE PRODUCTS. IN PARTICULAR, THEY WILL BE MADE AVAILABLE TO OTHER LABORATORIES WORKING ON EU CELL FACTORY PROGRAMS CONCERNED WITH THERMOPHILIC ORGANISMS. WE PLAN TO COMPLETE THE S. SOLFATARICUS GENOME, WHICH IS OF CONSIDERABLE INTEREST TO INDUSTRY BECAUSE OF THE THERMOSTABLE NATURE OF ALL ITS ENZYMES, AS A JOINT EUROPEAN CANADIAN EFFORT BY THE END OF THE THREE YEAR PROGRAM IN DECEMBER 1999.

Funding Scheme

CSC - Cost-sharing contracts
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Coordinator

Kobenhavns Universitet
Address
83 H,soelvgade
1307 København K
Denmark

Participants (3)

Novo-Nordisk A/S
Denmark
Address
1Bs,novo Alle
2880 Bagsværd
UNIVERSITE DE PARIS-SUD XI
France
Address
Rue Georges Clemenceau 15, Bftiment 400
91405 Gometz La Ville
WAGENINGEN UNIVERSITY
Netherlands
Address
4,Hesselink Van Suchtelenweg 4
6703 CT Wageningen