The aim of the proposal is to answer pertinent questions regarding the detection and diversity of methane oxidizing bacteria in the environment. We propose to develop and test new molecular and biochemical techniques including phylogenetic and functional gene probes, DDGE electrophoresis, PLFA-analysis, and 14C-radiotracers.
These techniques will be integrated in improved isolation strategiec for apparent non-culturable bacteria.
The specific objectives of the project are:
- To develop molecular and biochemical techniques suitable for detection of methane oxidizing bacteria in environmental samples
- To determine the in situ diversity and population dynamics of methane oxidizers in relation to environmental conditions.
- To apply the newly developed molecular and biochemical techniques in the isolation and characterization of novel methane oxidizers
- To generate a sequence database with environmental DNA and RNA libraries for methane and ammonia oxidizers.
The project has been divided into three research tasks that focuses on a. The diversity of high and low affinity methane oxidizers in soils (forest, agricultural, and rice paddy fields), b. The in situ population dynamics under changing environmental conditions, and c.
Isolation and characterization of novel methane oxidizers from selected environments (e.g. soils and marine systems).
Funding SchemeCSC - Cost-sharing contracts