Objective Bacillus subtilis is a model organism for Gram-positive bacteria including pathogens, e.g. Staphylococcus, Streptococcus, Corynebacterium, Clostridium and Listeria. It is also an important industrial organism. The determination of the sequence of its genome is therefore an important objective. An international agreement has been reached allowing the allocation of regions of the chromosome among the groups of the European and Japanese Bacillus subtilis genome sequencing networks, also including two biotechnology companies (Genencor International and Novo Nordisk). The European Bacillus subtilis genome sequencing project has been supported in the framework of the current Biotechnology contracts BIO2-CT93-0272 and BIO2-CT942011 which will end in December 1996. A relational database containing more than 2000 kbp of non-redundant Bacillus subtilis DNA sequence (1050 kbp has been contributed by the European network), representing about 60 % of the total genome, has been constructed at the Institut Pasteur. During the last two years the results of this European project have been presented at 13 international meetings, and have been published in several international journals, in particular in a special issue of the journal Microbiology (1995, 141: 249-350). A major difficulty encountered during the project stems from the unavailability of a simple cloning strategy for the construction of gene banks representative of the entire Bacillus subtilis chromosome. This is, at least in part, due to the generally high level of expression of Bacillus subtilis genes observed in Escherichia coli, leading to toxic effects in this commonly used cloning host. However, participating groups have employed a variety of strategies to overcome this limitation, including cloning into a range of plasmid, lambda and YAC vectors, genome walking experiments and in-vitro amplification using long-range PCR and inverse PCR techniques. Bacillus subtilis is also a major source of industrial enzymes. The world market (estimated at 400 million dollars in 1993) is dominated by the two companies mentioned above (Novo Nordisk and Genencor). An industrial platform has been set up to facilitate contacts between project participants and European biotechnology companies. The following companies have agreed in principle to this mode of cooperation: Novo Nordisk (Denmark, USA), Genencor (Finland, USA) and its subsidiary Gist-brocades (The Netherlands), F. Hoffmann La Roche AG (Switzerland), Eniricerche (Italy), Puratos (Belgium). Thirteen laboratories and five companies, which have a solid experience in large-scale sequencing, agreed to participate in the present proposal. Its objective is the determination of the remaining sequence (estimated at 1800 kbp), leading to the completion of the entire Bacillus subtilis genome sequence within one year. Fields of science natural sciencesbiological sciencesmicrobiologybacteriologynatural sciencesbiological sciencesgeneticschromosomesnatural sciencesbiological sciencesgeneticsgenomesnatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsenzymesnatural sciencescomputer and information sciencesdatabasesrelational databases Programme(s) FP4-BIOTECH 2 - Specific research, technological development and demonstration programme in the field of biotechnology, 1994-1998 Topic(s) 020102 - B. subtilis genome Call for proposal Data not available Funding Scheme CSC - Cost-sharing contracts Coordinator INSTITUT PASTEUR EU contribution No data Address 25,Rue du Docteur Roux 28 75724 PARIS France See on map Total cost No data Participants (14) Sort alphabetically Sort by EU Contribution Expand all Collapse all AGOWA - Gesellschaft fur Molekularbiologische Technologie mbH Germany EU contribution No data Address 185,Glienicker Weg 185 12489 Berlin See on map Total cost No data GATC - Gesellschaft fur Analyse-Technik und Consulting mbH Germany EU contribution No data Address 23,Fritz-Arnold-Str. 23 78467 Konstanz See on map Total cost No data Genotype GmbH, Molekularbiologische und biotechnologische Forschung Germany EU contribution No data Address 39,Angelhofweg Wilhelmsfeld See on map Links Website Opens in new window Total cost No data Institut National de la Recherche Agronomique France EU contribution No data Address Domaine de Vilvert 78352 Jouy-en-Josas See on map Total cost No data International Centre for Genetic Engineering and Biotechnology Italy EU contribution No data Address Padriciano 99 34012 Trieste See on map Total cost No data QIAGEN GMBH Germany EU contribution No data Address 4,Max-Volmer-Strasse 4 40724 Hilden See on map Total cost No data SCIENTIFIC RESEARCH AND DEVELOPMENT GMBH Germany EU contribution No data Address 43,Oberurseler Strasse 43 61440 Oberursel See on map Total cost No data THE PROVOST, FELLOWS AND SCHOLARS OF THE COLLEGE OF THE HOLY AND UNDIVIDED TRINITY OF QUEEN ELIZABETH NEAR DUBLIN HEREINAFTER TRINITY COLLEGE DUBLIN Ireland EU contribution No data Address Smurfit Institute,Trinity College 2 DUBLIN See on map Total cost No data UNIVERSITA DEGLI STUDI DI PAVIA Italy EU contribution No data Address Via Ferrata 1 27100 PAVIA See on map Total cost No data UNIVERSITE CATHOLIQUE DE LOUVAIN Belgium EU contribution No data Address 2/20,Place Croix du Sud 2/20 1348 LOUVAIN-LA-NEUVE See on map Total cost No data UNIVERSITY OF GRONINGEN Netherlands EU contribution No data Address 30,Kerklaan 30 9750 AA HAREN GN See on map Total cost No data UNIVERSITY OF NEWCASTLE UPON TYNE United Kingdom EU contribution No data Address Framlington Place NE2 4HH Newcastle - Upon Tyne See on map Total cost No data Università degli Studi di Padova Italy EU contribution No data Address Via Trieste 75 35121 Padova See on map Total cost No data Université de Lausanne Switzerland EU contribution No data Address 19,Rue Cesar-Roux 1005 Lausanne See on map Total cost No data