Objective
Peroxidases are of strategic importance to industry because of their numerous applications, including: biosensor construction, diagnostics, food processing, remediation of waste water, decolourization of pulp paper and textiles so avoiding the use of chlorine, and in dye transfer inhibition (bio bleaching).
This project's objective is to design 'improved' peroxidase enzymes by a combination of protein engineering and chemical modification techniques with: (1) increased stability towards heat, organic solvents and denaturants. (2) greater resistance to catalytic inactivation and damage by radical products. (3) enhanced activity during steady-state catalytic and chemiluminescent turnover.
We will study four peroxidases with important commercial applications or potential: The most used cationic horseradish peroxidase (HRP C), Coprinus cinereus peroxidase (CIP), soybean peroxidase (SBP) and anionic horseradish peroxidase (HRP A2).
Scientifically the project will: (1) study the factors at the protein level which control the stability and unfolding of these enzymes, in particular the role of Ca in stabilising enzyme structures.
(2) Determine new peroxidase crystal structures. (3) Determine the structure of their high oxidation state intermediates using fast x-ray /
Microspectrophotometer methodology. resonance raman and NMR spectroscopy will support these investigations (1)-(3).
The industrial participants, Ortho-Clinical Diagnostics Ltd and Novo Nordisk A/S, desire improved peroxidases for diagnostic and industrial enzyme applications. For new peroxidases which are found to offer a significant performance advantage, a business evaluation will be completed to assess the overall benefit of replacing current reagents with those based on the new `engineered' peroxidases.
The project will have the following objectives:
. More stable variant forms of HRP C and CIP obtained by a combination of protein engineering and chemical modification.
. Peroxidase variants with increased specific activities and enhanced chemiluminescent turnover.
. Crystal and solution structures of SBP and HRP A2.
. Structure and decay of peroxidase intermediates.
. Development of advanced time-resolved crystallographic methods. . Insight into enzyme stabilization by calcium ion.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences chemical sciences inorganic chemistry alkaline earth metals
- natural sciences physical sciences optics spectroscopy absorption spectroscopy
- natural sciences chemical sciences inorganic chemistry halogens
- natural sciences chemical sciences catalysis biocatalysis
- natural sciences biological sciences biochemistry biomolecules proteins enzymes
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Programme(s)
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Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
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Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
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Funding Scheme
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Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
9000 Ëlborg
Denmark
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.