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Content archived on 2024-04-30

Mechanisms for the regulation of Carotenoid production and accumulation in plants

Objective



The proposal utilises the skills of six laboratories to answer critical questions necessary for advancing our understanding of carotenoid formation and deposition in plants and to address the potential for its manipulation for agricultural, industrial and nutritional purposes. The work will focus upon increasing our knowledge of the biochemistry and regulation of carotenogenesis, its integration and inter-connection with other isoprenoid pathways in order to provide for the widespread manipulation of carotenoid production and accumulation in crop plants.
There are two objectives in the proposal:
1. To elucidate the regulatory mechanisms controlling carotenoid formation in green and non-green tissues of higher plants, encompassing the pathway from early precursors.
2. To clone and characterise novel structural genes and regulatory elements involved in carotenogenesis in plants.
The project has two integrated work packages, which utilise a range of higher plants in order to encompass the complexity of regulatory events thought to control carotenoid formation and deposition. A multi-disciplinary approach will be used, covering biochemistry, molecular biology, and analytical chemistry, with considerable use of plant transformations.
Workpackage 1 deals with elucidation of the mechanisms controlling carotenogenesis and accumulation, including regulation of gene expression, compartmentation of enzymes and substrates, properties of enzymes, metabolic fluxes, inter-pathway cross-talk and the influence of storage proteins on carotenoid accumulation. It also aims to establish the presence and importance of an alternative pathway to isopentenyl pyrophosphate. Workpackage 2 incorporates cloning and characterization of novel structural genes and regulatory elements in chromoplast and chloroplast-containing tissues, with widespread use of transgenic plants.

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Coordinator

ROYAL HOLLOWAY AND BEDFORD NEW COLLEGE
EU contribution
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Address
Egham Hill
TW20 0EX EGHAM,SURREY
United Kingdom

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Participants (5)

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